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ISSN 1995-5537

2 2010

«Biotechnology» journal, Vol. 3, No. 2, 2010
Р. 62 - 70, Bibliography. 35,  Russian.
Universal Decimal classification: 576.371:57.083

Institute for Problems of Cryobiology and Cryomedicine of National Academy of Sciences of Ukraine, Kharkiv
Device and conditions for alginate microbead generation are described. Methods for cell viability evaluation in alginate hydrogel are discussed. Encapsulation procedure was shown to have no significant influence on mesenchymal stromal cell (MSC) viability. It was found that the cells cultivated in alginate microbeads during 8 days maintained viability and metabolic activity however it was accompanied with proliferation stoppage. MSC released from alginate hydrogel were able to adhere to cultural plastic and renew proliferation. The results obtained allow to consider alginate hydrogel as biocompatible material for encapsulation of MSC. Therefore MSC encapsulated in alginate microbeads can be applied to cell technologies and tissue engineering.

Key words: alginate, alginate microbeads, encapsulation, mesenchymal stromal cells, cultivation.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008