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ISSN 1995-5537

2 2010

«Biotechnology» journal, Vol. 3, No. 2, 2010
Р. 42 - 47, Bibliography. 8, Ukrainian.
Universal Decimal classification: 577.152.34:577.151.5


Matselyukh O. V.

Institute of Microbiology and Virology of  the National Academy of Science of Ukraine, Kyiv

Сhemical mutagenesis of producer strain of Bacillus sp. 27 elastolytic enzymes was analysed using N-methyl-N'-nitro-N-nitrosoguanidine. 2.1% among a 1 000 of the investigated mutant variants shown a sign of elastase overactivity and only 7 variants proved to be stable during 6 months. Level of elastase activity for the selected variants was 31–100% higher as compared to the initial culture. It was found that one of the obtained Bacillus sp. 27 (88m) mutants was distinguished from the native culture in enzymes biosynthesis and in such physical and chemical indexes as enzyme complex dependence on pH and temperature of medium as well. It was shown that mutant 88m produced only one serine protease with elastolytic action which molecular mass was about 28,7 kDa. Synthesis of this enzyme was twice as much as in comparison with the native culture.

Key words: Вacillus sp., elastase, mutants, N-methyl-N'-nitro-N-nitrosoguanidine.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008