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ISSN 1995-5537

2 2010

« Biotechnology» journal, Vol. 3, No. 2, 2010
Р. 33 - 41, Bibliography. 9, Ukrainian..
Universal Decimal classification: 577.115.7:579.842.1/2

Zabolotny Institute of Microbiology and Virology of National Academy of Sciences of Ukraine, Kyiv
I. I. Mechnikov Odessa National University
I. I. Mechnikov Institute of Microbiology and Immunology of Academy of Medical Sciences of Ukraine, Kharkiv
The results of the comparative toxicity studies of native and modified with complex compounds of germanium or tin lipopolysaccharides (LPS) of Rahnella aquatilis strains 95U004, 95U011 and 96U035 indicate toxicity decreased of all investigated LPS due to modification of LPS with complexes of germanium (IV) with nicotinoylhydrazon salicylic aldehyde.

Interaction of LPS with the tin (IV) complex with nicotinoylhydrazon salicylic aldehyde led to decrease in toxicity only LPS R. aquatilis 95U004. In the study of pyrogenic actions of native and modified LPS there was not found out a certain regularity in the action of modifying agents most of which caused a reduction of pyrogenic activity. Almost all the modifications do not affect the neutralizing activity of LPS of the three investigated strains R. aquatilis 95U004, 95U011 and 96U035, in homologous and in heterologous systems.

Modified complex tin (IV) with nicotinoylhydrazon salicylic aldehyde LPS R. aquatilis 95U004 that had lost serological activity toward antiserum to the type strain R. aquatilis ATCC 33071 and modified complex of germanium (IV) with nicotinoylhydrazon salicylic aldehyde and complex tin (IV) with 2-aminobenzoylhydrazon salicylic aldehyde LPS R. aquatilis 96U035 that had lost their serological activity of both homologous and heterologous systems with antiserum to other representatives of the third serogroup were exceptions.

Key words: lipopolysaccharide, Rahnella aquatilis, complex compounds of germanium and tin, toxicity, pyrogenicity.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008