SSN 2410-7751 (Print)
ISSN 2410-776X (Online)

Biotechnologia Acta Т. 18, No. 2, 2025
P.  , Bibliography 4 , Engl.

UDC 615.262.1:615.454.1].07:616.147.17-007.64-085
EFFICACY OF COMBINED RECTAL CREAM “DILATIL” (DILTIAZEM/LIDOCAINE/METHYL-URACIL) IN PRE-CLINICAL STUDY ON A MODEL OF ACUTE COMPLICATED ANAL FISSURE IN COMPARISON WITH RECTAL CREAM NIFECAIN

G.V. ZAYCHENKO, M.P. TYMOFIEIEV
1Pharmacology department of the Bogomolets National Medical University, Kyiv.
Abstract. Abstract. Aim: to assess efficacy of a new rectal cream “Dilatil” (diltiazem/lidocaine/methyluracil) (D) in pre-clinical study on a model of acute complicated anal fissure (ACAF) in compared to rectal cream “Nifecain” (nifedipine/lidocaine) (N).
Abstract. Aim: to assess efficacy of a new rectal cream “Dilatil” (diltiazem/lidocaine/methyluracil) (D) in pre-clinical study on a model of acute complicated anal fissure (ACAF) in compared to rectal cream “Nifecain” (nifedipine/lidocaine) (N)..
Materials and Methods: animal model of anal fissure, macroscopic assessment, enzyme-linked immunosorbent assay, statistical Student’s and Wilcoxon’s tests.
Results: At the end of the study in the D group, the severity of the pathological process was statistically lower than in the Control pathology (CP) and N groups. Levels of IL-6, IL-10, and TNF-α in the homogenate of anodermal tissue (HAT) in the D group were significantly lower compared to CP. The level of IL-6 was significantly lower in the D group compared to N.
Conclusions: better efficacy of D vs N was observed in a pre-clinical study that was also confirmed by the level of IL-10 in HAT tissue at the end of the experiment.
Keywords. Rectal cream, anal fissure, Dilatil, Nifecain, macroscopic assessment, enzyme-linked immunosorbent assay, IL-10, IL-6, HIF-1α, TNF-α, calcium channel blockers, diltiazem.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2025