LIMITATIONS OF HUMAN LEUKOCYTE ANTIGEN MOLECULAR TYPING METHODS FOR PATIENTS WITH ACUTE MYELOID LEUKEMIA RELAPSE AFTER HEMATOPOIETIC STEM CELL TRANSPLANTATION L.O. SISHCHUK, I.I. TUBALTSEVA, N.A. TSOKOLENKO, A.O. MAKSYMCHUK, A. V. KUKHOL, V.V. ANDRUSENKO

Details: Hits: 5

SSN 2410-7751 (Print)
ISSN 2410-776X (Online)

cover biotech acta general

Biotechnologia Acta Т. 18, No. 2, 2025
P. , Bibliography 4 , Engl.
UDC 616.155.392-089.843:611.018.46+616-097
LIMITATIONS OF HUMAN LEUKOCYTE ANTIGEN MOLECULAR TYPING METHODS FOR PATIENTS WITH ACUTE MYELOID LEUKEMIA RELAPSE AFTER HEMATOPOIETIC STEM CELL TRANSPLANTATION

L.O. SISHCHUK, I.I. TUBALTSEVA, N.A. TSOKOLENKO, A.O. MAKSYMCHUK, A. V. KUKHOL, V.V. ANDRUSENKO, A.O. MAZANOVA,
Y.A. HROHUL
National Specialized Children's Hospital "OKHMATDYT" of the Ministry of Health of Ukraine, Kyiv

Aim. The work purposed to compare molecular typing methods with different resolutions in clinical cases without loss of heterozygosity (LOH) in HLA genes.
Methods. HLA typing was performed using molecular techniques based on sequence-specific primers (SSP) or high-resolution sequencing (NGS and Sanger sequencing).
Results. the presence of an extra allelic variant in testing DNA samples was determined only with the low-resolution SSP method. At the same time, any type of sequencing did not allow for distinguishing donors’ alleles from patients.
Conclusion. Despite the high sensitivity of NGS and Sanger sequencing, the use of these methods is associated with difficulties in accurately determining the absence of HLA-LOH.
Keywords: HLA-LOH, HLA typing, hematopoietic stem cell transplantation, sequencing.