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ISSN 2410-776X (Online)
ISSN 2410-7751 (Print)

 6 2018

Biotechnologia Acta, V. 11, No 6, 2018
Р. 47-54, Bibliography 25, English
Universal Decimal Classification: 57.023: 58.039


N. B. Golub1, M. Tsvetkovych1, I. I. Levtun1, V. I. Maksyn2

1National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute”
2National University of Life and Environmental Sciences of Ukraine, Kyiv

The aim of the research was to study the development of Chlorella vulgaris at culturing on the modified Gromov 6 medium with high concentrations of nanostructured ferric citrate and also its effect on photosynthesis pigments accumulation. It was demonstrated that the highest intracellular iron content (15 mg/g of dry mass) in the culture cells was typical with nanostructured ferric citrate content of 30 mg/dm3 of culture medium, the highest content of chlorophyll a — 23 mg/g of dry algae mass, b — 7.5 mg/g of dry mass, and carotenoids — 9.2 mg/g of dry mass was observed at nanostructured ferric citrate content of 20 mg/dm3. The use of nanostructured ferric citrate leaded to an increase in the chlorella biomass yield by 3 times with compared to standard technology. Simultaneously, intracellular iron content in cells increased significantly with the use of nanostructured ferric citrate, which increases their value as a nutritional supplement. In order to increase the biomass yield and intracellular iron content in cells, application of nanostructured ferric citrate is recommended.

Key words: chlorella cells, biomass, intracellular iron, chlorophylls, carotenoids, nanostructured ferric citrate.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2018