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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

 1 2016

"Biotechnologia Acta" V. 9, No 1, 2016
Р. 48-54, Bibliography 21, English
Universal Decimal Classification: 579.63:577.29


D. R. Аbdulina 1, L. М. Purish 1, G. А. Iutynska 1, М. М. Nikitin 2, A. G. Golikov 2

1 Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, Кyiv
2 LLC GenBit, Moscow, Russian Federation

The possibility of the designing test-systems for specific detection of corrosive-relevant sulfate-reducing bacteria using real-time PCR assay were investigated. This method of the bacteria identification is based on the detection of the functional genes, encoding key enzymes of dissimilatory sulfate-reduction pathway, i.e. dissimilatory sulfitreductase α subunit dsrA. It was established among the six test-systems specificity reveal only three designed on the base of Desulfotomaculum, Desulfovibrio, Desulfobulbus genera sequences. The most corrosive-relevant strain Desulfovibrio sp. UCM B-11503 dsrA gene detected more effectively (threshold cycle was 20,0), than less corrosive-relevant strains Desulfovibrio sp. UCM B-11504 (threshold cycle was 28,1) and for Desulfotomaculum sp. UCM B-11505 and Desulfomicrobium sp. UCМ B-11506 were 24.9 and 23.1 cycles, respectively. Test-systems allowed identifying corrosive-relevant sulfate-reducing bacteria faster and more effective. This approach will serve as a base for monitoring of these bacteria for estimating corrosion sites on the high-level dangerous man-caused objects.

Кey words: sulfate-reducing bacteria, dissimilatory sulfate-reduction genes, test-systems, real-time PCR.

© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2016