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ISSN 1995-5537

3 2012

"Biotechnology" journal V. 5, No. 3, 2012
Р. 105 - 111, Bibliography 20, Russian.
Universal Decimal classification: 582.232:[581.143+577.122.5]


А. B. Borovkov, І. M. Gudvilovych

Institute of Biology of Southern Seas of National Academy of Sciences of Ukraine, Sevastopol

The dynamics of biomass density, chlorophyll а and chlorophyll b, total carotenoids and protein content of Dunaliella salina semicontinuous culture were investigated. The type of change of pigments and protein content of D. salina was determined under semicontinious cultivation; the possibility of regulation of these microalgae chemical composition with the help of varying the specific flow rate was shown. Maximum content of pigments of D. salina biomass was noticed under specific flow rate 0.14 day-1, and it decreased while increasing specific flow rate from 0.14 to 0.42 day-1. The production characteristics of D. salina semicontinuous culture on biomass, chlorophyll a, chlorophyll b, total carotenoids and protein were defined. It was proved that the D. salina productivity under the batch cultivation 1.2 – 2.1 times less than under the semicontinious cultivation. Maximum pigments, biomass and protein productivity was realized under specific flow rate 0.32 day-1 and reached 0.45 g•l-1•day-1 of organic substances in biomass, 4.0 mg•l-1•day-1 in carotenoids and 0.25 g•l-1•day-1 in protein. There was drawn a comparison between the got culture productivity under the laboratory and industrial conditions for the Ukrainian and world companies. The areas for carrying the researches out were defined for the increasing the D. salina culture productivity under the industrial conditions.

Key words: Dunaliella salina, semicontinuous cultivation, pigments, productivity.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008