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ISSN 1995-5537

3 2010

"Biotechnology" journal Vol. 3, No. 3, 2010
Р. 78-83, Bibliography 26, English.
Universal Decimal classification: 582.28 : 631.466

Science Research Center SFA, 7308 Hwy 59N, Nacogdoches, TX 75965, USA
The study compared the composition of arbuscular mycorrhizal fungal (AMF) communities colonizing the rootsof two common native plants along a slope-related moisture gradient on a small hillside in a natural east Texas (USA) forest. PCR amplification of a region of the small subunit ribosomal 18S RNA gene with Glomeromycota-specific primers was used to detect AMF in the roots of Chasmanthium sessiliforum (Poir.) Yates and Callicarpa americana L. The PCR products were subjected to denaturing gradient gel electrophoresis (DGGE) to obtain community profiles. Comparison of profiles revealed significant differences between the AMF communities associated with the two hosts. Additionally, communities of AMF colonizing C. sessiliforum differed according to their location along the hill slope gradient.

Key words
Arbuscular mycorrhizal fungi, denaturing gradient gel electrophoresis, DGGE, AMF diversity, 18S rRNA, multivariate analysis.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008