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ISSN 1995-5537

1 2009

"Biotechnology" journal Vol. 2, No. 1, 2009
Р. 69-79, Bibliography 46, Ukrainian.
Universal Decimal classification: 57.083.13+577.152.321*1


O. I. Kubrak, V. I. Lushchak

Vassyl Stefanyk Precarpathian National University, Ivano-Frankivsk

Strain BKL40 was identified as a producer of thermostable alkaline α-amylase. Maximum production of this α-amylase was achieved by optimizing culture conditions. Production of α-amylase seemed to be independent on the presence of starch in the culture medium and was stimulated by peptone (0.3%, w/v) and yeast extract (0.2%, w/v). The enzyme was thermostable and retained amylolytic activity after 30 min of incubation at 60 and 70 0C. High activity was maintained over a broad pH range from 6.0 to 11.0 and the enzyme remained active after alkaline incubation for 24 h. Bacillus sp. BKL40 α-amylase was not stimulated by calcium and other bivalent metal cations at 1mM and was not inhibited by EGTA or EDTA at 1-10 mM, suggesting that this α-amylase does not contain calcium ions in its active site.

Key words: α-amylase, Bacillus sp., alkalostability, thermostability, production.