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ISSN 1995-5537

1 2009

"Biotechnology" journal Vol. 2, No. 1, 2009
Р. 107-116, Bibliography 61, Ukrainian.
Universal Decimal classification: 547.495.9+577.151.3+543.067+544.165

Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Kyiv

Determination of creatinine concentrations in biological fluids is an increasingly important clinical test. This analyte is used for evaluation of renal function and muscle damage. Today there are plenty of methods of creatinine detection, however only some of them could be used for the biomedical analysis. The colorimetric method based on Jaffe reaction is the most widely used one for the creatinine analysis. The main disadvantage of this method is dependence on the presence of possible interferents that are present in the biological samples. This leads to the increased amount of pseudo-positive results. Special attention in the review is paid to the most effective methods of the metabolite detection (enzyme- and synthetic receptor-based biosensors). The advantages of these methods are small volumes of the analyzed samples, specificity, inexpensive equipment and fast time of analysis.

Key words: creatinine, analysis, biosensors, molecularly-imprinted polymers.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008