6_2018(en)

Details: Hits: 49

ISSN 2410-776X (Online)
ISSN 2410-7751 (Print)

6 2018

Biotechnologia Acta, V. 11, No 6, 2018
https://doi.org/10.15407/biotech11.06.067
Р. 67-72, Bibliography 21, English
Universal Decimal Classification: 616-089.843:612.419-018.4

ABILITY OF THYMIC MSCs AND THEIR DERIVATIVES TO INTERACT WITH THE CELLS OF LYMPHOID ORIGIN

D. L. Demchenko

State Institute of Genetic and Regenerative Medicine of the National Academy of Medical Sciences of Ukraine, Kyiv

The aim of the research was to determine the ability of thymic multipotent stromal cells and their derivatives to interact with lymphocytes obtained from different sources. It was shown that a part of thymic cells from 6?8-weeks-old С57BL mice in vitro were characterized by such properties: the ability to adhere to the surfaces of cell-culture plastic, the specific fibroblast-like morphology, and the ability to directed adipogenic and osteogenic differentiation. Due to these properties, the cell populations isolated from thymus could be attributed to the multipotent mesenchymal stromal cells (MMSC) or mesenchymal stem cells (MSCs). We have shown that all types of stromal cells have an ability to interact with the lymphoid cells obtained from different sources (thymocytes, splenocytes, cells of the lymph nodes and bone marrow). The largest number of intercellular associations has been formed with the thymocytes, and the smallest one – with the lymphoid cells of bone marrow. Among differentiated forms osteogenic cells are capable to create higher number of intercellular associations, as compared to adipocytes. Thus, probably the intercellular contact interactions between the MSCs and hematopoietic cells might be used as one of the new approaches for efficient and directed modification of the cell properties.

Key words: mesenchymal stem cells from thymus, MSCs, differentiation, lymphoid cells, intercellular contacts.

References

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2. Abumaree M. H., Abomaray F. M., Alshabibi M. A., AlAskar A. S., Kalionis B. Immunomodulatory properties of human placental mesenchymal stem/stromal cells. Placenta. 2017, V. 59, P. 87–95. https://doi.org/10.1016/j.placenta.2017.04.003

3. El-Kehdy H., Pourcher G., Zhang W., Hamidouche Z., Goulinet-Mainot S., Sokal E. Hepatocytic Differentiation Potential of Human Fetal Liver Mesenchymal Stem Cells: In Vitro and In Vivo Evaluation. Stem Cells Int. 2016, V. 2016, P. 6323486. https://doi.org/10.1155/2016/6323486

4. Wang S., Mundada L., Johnson S., Wong J., Witt R., Ohye R. G. Characterization and angiogenic potential of human neonatal and infant thymus mesenchymal stromal cells. Stem Cells Transl. Med. 2015, V. 4, P. 339–350. https://doi.org/10.5966/sctm.2014-0240

5. Arai F. Self-renewal and differentiation of hematopoietic stem cells. Rinsho Ketsueki. 2016, V. 57, P. 1845–1851. https://doi.org/10.11406/rinketsu.57.1845

6. Wang S., Mundada L., Colomb E., Ohye R. G., Si M-S. Mesenchymal Stem/Stromal Cells from Discarded Neonatal Sternal Tissue: In Vitro Characterization and Angiogenic Properties. Stem Cells Int. 2016, V. 2016, P. 5098747. https://doi.org/10.1155/2016/5098747

7. Banwell C. M., Partington K. M., Jenkinson E. J., Anderson G. Studies on the role of IL-7 presentation by mesenchymal fibroblasts during early thymocyte development. Eur. J. Immunol. 2000, V. 30, P. 2125–2129. https://doi.org/10.1002/1521-4141(2000)30 https://doi.org/10.1002/1521-4141(2000)30 https://doi.org/10.1002/1521-4141(2000)30 https://doi.org/10.1002/1521-4141(2000)30:8<2125::AID-IMMU2125>3.0.CO;2-H

8. Chung B., Montel-Hagen A., Ge S., Blumberg G., Kim K., Klein S. Engineering the human thymic microenvironment to support thymopoiesis in vivo. Stem Cells. 2014, V. 32, P. 2386–2396. https://doi.org/10.1002/stem.1731

9. Suniara R. K., Jenkinson E. J., Owen J. J. An essential role for thymic mesenchyme in early T cell development. J. Exp. Med. 2000, V. 191, P. 1051–1056. PMID: 10727466 https://doi.org/10.1084/jem.191.6.1051

10. Nikolskiy I. S., Nikolskaya V. V., Savinova V. O. Intravenous injection of multipotent stromal cells of thymus and immune response. J. Med. Acad. Sci. 2012, V. 4, P. 55–56. (In Russian).

11. Barda-Saad M., Rozenszajn L. A., Globerson A., Zhang A. S., Zipori D. Selective adhesion of immature thymocytes to bone marrow stromal cells: relevance to T cell lymphopoiesis. Exp. Hematol. 1996, V. 24, P. 386–391. PMID:8641370

12. Lotfinegad P., Shamsasenjan K., Movassaghpour A., Majidi J., Baradaran B. Immunomodulatory nature and site specific affinity of mesenchymal stem cells: a hope in cell therapy. Adv. Pharm. Bull. 2014, V. 4, P. 5–13. https://doi.org/10.5681/apb.2014.002

13. Li W., Ren G., Huang Y., Su J., Han Y., Li J. Mesenchymal stem cells: a double-edged sword in regulating immune responses. Cell Death Differ. 2012, V. 19, P. 1505–1513. https://doi.org/10.1038/cdd.2012.26

14. Le Blanc K., Mougiakakos D. Multipotent mesenchymal stromal cells and the innate immune system. Nat. Rev. Immunol. 2012, V. 12, P. 383–396. https://doi.org/10.1038/nri3209

15. Nikolskiy I. S., Nikolskaya V. V., Demchenko D. L., Zubov D. O. Potentiation of directed osteogenic differentiation of thymic multipotent stromal cells by prior co-cultivation with thymocytes. Cell Organ Transplantol. 2016, V. 4. https://doi.org/10.22494/cot.v4i2.59

16. Prockop D. J., Bunnell B. A., Phinney D. G., editors. Mesenchymal Stem Cells. Totowa, NJ: Humana Press. 2008. https://doi.org/10.1007/978-1-60327-169-1

17. Vikulin I. M., Gorbachev V. E., Korobitsin B. V. Assessing the suitability measurements and elimination of abnormal values. Naukovі Pratsі ONAZ Іm O. S. Popova – Proceedings of the O. S. Popov ОNAT. 2007, V. 2, P. 106–111. (In Ukrainian).

18. Dominici M., Le Blanc K., Mueller I., Slaper-Cortenbach I., Marini F., Krause D. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006, V. 8, P. 315–317. https://doi.org/10.1080/14653240600855905

19. Pittenger M. F., Mackay A. M., Beck S. C., Jaiswal R. K., Douglas R., Mosca J. D. Multilineage potential of adult human mesenchymal stem cells. Science. 1999, V. 284, P. 143–147. PMID: 10102814 https://doi.org/10.1126/science.284.5411.143

20. Siepe M., Thomsen A. R., Duerkopp N., Krause U., F?rster K., Hezel P. Human neonatal thymus-derived mesenchymal stromal cells: characterization, differentiation, and immunomodulatory properties. Tis. Eng. Part A. 2009, V. 15, P. 1787–1796. https://doi.org/10.1089/ten.tea.2008.0356

21. Nikolska K. I. Peculiarities of Culture and In vitro Contact Interaction of Cryopreserved Thymic Multipotent Stromal Cells and Hemopoietic Cells. Probl. Cryobiol. Cryomed. 2018, V. 28, P. 005–0013. https://doi.org/10.15407/cryo28.01.005

Details: Hits: 89

ISSN 2410-776X (Online)
ISSN 2410-7751 (Print)

6 2018

"Biotechnologia Acta" V. 11, No 6, 2018
https://doi.org/10.15407/biotech11.06.082
Р. 82-91, Bibliography 37, English
Universal Decimal Classification: 579.663

THE PROPERTIES OF SURFACTANTS SYNTHESIZED BY Acinetobacter calcoaceticus ІMV В-7241 ON REFINED AND WASTE SUNFLOWER OIL

T. P. Pirog, D. A. Lutsai, S. I. Antonuk, I. V. Elperin

National University of Food Technologies, Kyiv, Ukraine

The aim of the work was to compare the antimicrobial and anti-adhesive activity (including the ability to destroy biofilms), as well as the effect on oil degradation of the surfactants synthesized by the culture of Acinetobacter calcoaceticus IMV B-7241 on refined waste sunflower oil.

The surfactants were extracted from supernatant of cultural liquid by mixture of chloroform and methanol (2:1). The number of attached cells and the degree of biofilm destruction were analyzed spectrophotometrically. Antimicrobial properties of the surfactants were determined by index of the minimal inhibiting concentration (MIC). The concentration of oil in water was analyzed by the gravimetric method after extraction with hexane.

It was shown that surfactants synthesized in medium with 2% of both refined and waste oil were characterized by high antimicrobial (MIC with respect to bacterial test cultures 0.8–29 μg/ml, Candida albicans D-6 26 — 58 μg/ml) and anti-adhesive (decreasing number of bacterial and fungal cells of test cultures attached to abiotic surfaces by 35–70%, destruction of biofilms by an average of 40–44%) activity. Increasing concentration of waste oil in the medium to 4% was accompanied by the formation of surfactants with low antimicrobial activity, in the presence of which the degree of oil destruction in water (3–6 g/l) was 80–88% in 20 days, which is 10–16% higher than when using surfactants synthesized in a medium with 2% oil.

The obtained data indicate on the need for studies on the effect of cultivation conditions of producer on the properties of synthesized surfactants for the production of final product with stable predetermined properties, depending on the field of practical application.

Key words: microbial surfactants, waste oil, antimicrobial and anti-adhesive activity, oil destruction.

References

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2. Paulino B. N., Pess?a M. G., Mano M. C., Molina G., Neri-Numa I. A., Pastore G. M. Current status in biotechnological production and applications of glycolipid biosurfactants. Appl. Microbiol. Biotechnol. 2016, 100 (24), 10265–10293. https://doi.org/10.1007/s00253-016-7980-z

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4. Franco Marcelino P. R., da Silva V. L., Rodrigues Philippini R., Von Zuben C. J., Contiero J., Dos Santos J. C., da Silva S. S. Biosurfactants produced by Scheffersomyces stipitis cultured in sugarcane bagasse hydrolysate as new green larvicides for the control of Aedes aegypti, a vector of neglected tropical diseases. PLoS One. 2017, 12 (11), e0187125. https://doi.org/10.1371/journal.pone.0187125

5. Parthipan P., Preetham E., Machuca L. L., Rahman P. K., Murugan K., Rajasekar A. Biosurfactant and degradative enzymes mediated crude oil degradation by bacterium Bacillus subtilis A1. Front. Microbiol. 2017, V. 8, P. 193. https://doi.org/10.3389/fmicb.2017.00193

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7. Chong H., Li Q. Microbial production of rhamnolipids: opportunities, challenges and strategies. Microb. Cell Fact. 2017, 16 (1), 137. https://doi.org/10.1186/s12934-017-0753-2

8. Ebadipour N., Lotfabad T. B., Yaghmaei S., RoostaAzad R. Optimization of low-cost biosurfactant production from agricultural residues through response surface methodology. Prep. Biochem. Biotechnol. 2016, 46 (1), 30–38. https://doi.org/10.1080/10826068.2014.979204

9. Pirog T. P., Shulyakova M. O., Nikituk L. V., Antonuk S. I., Elperin I. V. Industrial waste bioconversion into surfactants by Rhodococcus erythropolis ІMV Ас-5017, Acinetobacter calcoaceticus ІMV В-7241 and Nocardia vaccinii ІMV В-7405. Biotechnol. acta. 2017, 10 (2), 22–33. https://doi.org/10.15407/biotech10.02.022

10. Almeida D. G., Soares da Silva R. C., Luna J. M., Rufino R. D., Santos V. A., Sarubbo L. A. Response surface methodology for optimizing the production of biosurfactant by Candida tropicalis on industrial waste substrates. Front. Microbiol. 2017, V. 8, P. 157. https://doi.org/10.3389/fmicb.2017.00157

11. Pirog T. P., Sidor I. V., Lutsai D. A. Calcium and magnesium cations influence on antimicrobial and antiadhesive activity of Acinetobacter сalcoaceticus ІMV B-7241 surfactants. Biotechnol. acta. 2016, 9 (6), 50–57. https://doi.org/10.15407/biotech9.06.050

12. Pirog T. P., Nikituk L. V., Shevchuk T. A. Influence of divalent cations on synthesis of Nocardia vaccinii ІMV B-7405 surfactants with high antimicrobial and anti-adhesion activity. Mikrobiol. Zh. 2017, 79 (5), 13–22. (In Ukrainian).

13. De Rienzo M. A., Martin P. J. Effect of mono and di-rhamnolipids on biofilms preformed by Bacillus subtilis BBK006. Curr. Microbiol. 2016, 73 (2), 183–189. https://doi.org/10.1007/s00284-016-1046-4

14. Kim K., Lee Y., Ha A., Kim J. I., Park A. R., Yu N. H., Son H., Choi G. J., Park H. W., Lee C. W., Lee T., Lee Y. W., Kim J. C. Chemosensitization of Fusarium graminearum to chemical fungicides using cyclic lipopeptides produced by Bacillus amyloliquefaciens strain JCK-12. Front. Plant Sci. 2017, V. 8, P. 2010. https://doi.org/10.3389/fpls.2017.02010

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16. Ramachandran R., Shrivastava M., Narayanan N. N., Thakur R. L., Chakrabarti A., Roy U. Evaluation of antifungal efficacy of three new cyclic lipopeptides of the class bacillomycin from Bacillus subtilis RLID 12.1. Antimicrob. Agents Chemother. 2018, V. 62, P. e01457-17. https://doi.org/10.1128/AAC.01457-17

17. Pirog T. P., Lutsai D. A., Shevchuk T. A., Iutynska G. O., Elperin I. V. Аntimicrobial and anti-adhesive activity of surfactants synthesized by Acinetobacter calcoaceticus IMV V-7241 on technical glycerol. Mikrobiol. Zh. 2018, 80 (2), 14–27. (In Ukrainian). https://doi.org/10.15407/microbiolj80.02.014

18. Pirog T. P., Nikituk L. V., Antonuk S. I., Shevchuk T. A., Iutynskaya G. A. Intensification of Acinetobacter calcoaceticus IMV B-7241 surfactants synthesis on waste sunflower oil. Mikrobiol. Zh. 2018, 80 (1), 15–26. (In Russian). https://doi.org/10.15407/microbiolj80.01.015

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23. Pirog T. P., Shevchuk T. A., Voloshina I. N., Gregirchak N. N. Use of claydite-immobilized oil-oxidizing microbial cells for purification of water from oil. Appl. Biochem. Microbiol. 2005, 41 (1), 51–55. https://doi.org/10.1007/s10438-005-0010-z

24. Pirog T., Sofilkanych A., Konon A., Shevchuk T., Ivanov S. Intensification of surfactants’ synthesis by Rhodococcus erythropolis IMV Ac-5017, Acinetobacter calcoaceticus IMV B-7241 and Nocardia vaccinii K-8 on fried oil and glycerol containing medium. Food Bioprod. Process. 2013, 91 (2), 149–157. https://doi.org/10.1016/j.fbp.2013.01.001

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27. Pantazaki A. A., Dimopoulou M. I., Simou O. M., Pritsa A. A. Sunflower seed oil and oleic acid utilization for the production of rhamnolipids by Thermus thermophilus HB8. Appl. Microbiol. Biotechnol. 2010, 88 (4), 939–951. https://doi.org/10.1007/s00253-010-2802-1

28. Rufino R. D., Luna J. M., Sarubbo L. A. Antimicrobial and anti-adhesive potential of a biosurfactant Rufisan produced by Candida lipolytica UCP 0988. Coll. Surf. B. Biointerfaces. 2011, 84 (1), 1–5. https://doi.org/10.1016/j.colsurfb.2010.10.045

29. Nitschke M., Costa S. G., Contiero J. Structure and applications of a rhamnolipid surfactant produced in soybean oil waste. Appl. Biochem. Biotechnol. 2010, 160 (7), 2066–2074. https://doi.org/10.1007/s12010-009-8707-8

30. Pirog Т. P., Panasyuk E. V., Nikityuk L. V., Iutinska G. O. Influence of cultivation conditions on antimicrobial properties of Nocardia vaccinii ІMV B-7405 surfactants. Biotechnol. acta. 2016, 9 (1), 38–47. https://doi.org/10.15407/biotech9.01.038

31. Pirog T. P., Nikituk L. V., Antonuk S. I., Shevchuk T. A., Iutynska G. O. Peculiarities of Nocardia vaccinii ІMV В-7405 surfactants synthesis on waste oil of different quality and their antimicrobial properties. Mikrobiol. Zh. 2017, 79 (2), 13–22. (In Ukrainian). https://doi.org/10.15407/microbiolj79.02.013

32. Pirog T. P., Nikituk L. V., Tymoshuk K. V., Shevchuk T. A., Iutynska G. O. Biological properties of Nocardia vaccinii IMV B-7405 surfactants synthesized on fried sunflower oil. Mikrobiol. Zh. 2016, 78 (2), 2–12. (In Ukrainian). http://microbiolj.org.ua/images/files/magazine/2016/2/2016_78_2_01_Pirog.pdf

33. Hajfarajollah H., Mokhtarani B., Noghabi K. A. Newly antibacterial and antiadhesive lipopeptide biosurfactant secreted by a probiotic strain, Propionibacterium freudenreichii. Appl. Biochem. Biotechnol. 2014, 74 (8), 2725–2740. https://doi.org/10.1007/s12010-014-1221-7

34. Pirog T. P., Savenko I. V., Lutsay D. A. Microbial surface-active substances as antiadhesive agents. Biotechnol. acta. 2016, 9 (3), 7–22, https://doi.org/10.15407/biotech9.03.007

35. Kiran G. S., Priyadharsini S., Sajayan A., Priyadharsini G. B., Poulose N., Selvin J. Production of lipopeptide biosurfactant by a marine Nesterenkonia sp. and its application in food industry. Front. Microbiol. 2017, V. 8, P. 1138. https://doi.org/10.3389/fmicb.2017.01138

36. Pirog T. P., Konon A. D., Savenko I. V. Microbial surfactants in environmental technologies. Biotechnol. acta. 2015, 8 (4), 21–39. https://doi.org/10.15407/biotech8.04.021

37. Whang L. M., Liu P. W., Ma C. C., Cheng S. S. Application of biosurfactants, rhamnolipid, and surfactin, for enhanced biodegradation of diesel-contaminated water and soil. J. Hazard. Mater. 2008, 151 (1), 155–163. https://doi.org/10.1016/j.jhazmat.2007.05.063

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ISSN 2410-776X (Online)
ISSN 2410-7751 (Print)

6 2018

Biotechnologia Acta, V. 11, No 6, 2018
https://doi.org/10.15407/biotech11.06.073
Р. 73-81, Bibliography 16, English
Universal Decimal Classification: 579.695

NATURAL AND SYNTHETIC SOLID CARRIERS IN FLOW MODULE FOR MICROBIAL SEWAGE FILTRATE PURIFICATION

O. B. Tashyrev, I. B. Sioma, G. O. Tashyreva, V. M. Hovorukha

Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, Kiyv

The aim of the research was to develop theoretic principles of an efficient biotechnology for microbial purification of concentrated sewage filtrate from a wide range of organic and non-organic compounds, and experimentally confirmed these principles. The experiment combined conventional microbiological, physical and chemical methods in which ten different types of solid carriers, both natural and artificial, were used. A comparative analysis of these types of solid carriers used in the flow system and a structured evaluation of treatment parameters was provided. The obtaining results demonstrated the effectiveness of the use of approach sewage microbial purification in a flow system. Developed principles can be used as a basis for new efficient biotechnologies for sewage microbial purification.

Key words: microbial purification, toxic filtrate, sewage, inert carriers.

References

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2. Gaidin M., Diakiv V. O., Pohrebennyk V. D., Pashuk A. V. Chemical content of the filtrate of Lviv landfill of solid domestic waste. Nature of west Polissia and the neighbouring territories: sci. pap. comp. Lutsk. 2013, N. 10, P. 43–50. (In Ukrainian).

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5. Sablii L. A. Anaerobic-aerobic treatment of high concentrated industrian sewages. Water and water treatment technologies. 2011, 1 (3). (In Russian).

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7. Tashyrev O. B., Tashyreva G. O. Method of removal of a wide spectrum of metals from water solutions with mixed microbial communities biomass. Patent of Ukraine 7C22B3/00, C02F1/00. 2014. (In Russian).

8. Ternes T. Challenges for the future: emerging micropollutants in urban water cycle. BFG, Koblenz, Germany (presentation for a seminar at UNESCO-IHE, Delft, the Netherlands). 2009.

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10. Malovanyy Myroslav, Moroz Oleksandr I., Hnatush Svitlana O., Maslovska Olga D., Zhuk Volodymyr, Petrushka Ihor M., Nykyforov Volodymyr, Sereda Andriy. Perspective Technologies of the Treatment of the Wastewaters with High Content of Organic Pollutants and Ammoniacal Nitrogen. J. Ecol. Eng. 2019, 20 (2), 8–15. https://doi.org/10.12911/22998993/94917

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15. Sioma I. B., Tashyrev A. B., Govorukha V. M., Prekrasna Y. P.Toxic Metals Extraction During Potato Fermentation. Ecol. Engin. Envir. Protec, 2017, N. 1, P. 6–67.

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ISSN 2410-776X (Online)
ISSN 2410-7751 (Print)

6 2018

Biotechnologia Acta, V. 11, No 6, 2018
https://doi.org/10.15407/biotech11.06.055
Р. 55-66, Bibliography 17, English
Universal Decimal Classification: 552.57:579.6

OPTIMIZATION OF THE COAL BACTERIAL DESULFURIZATION USING MATHEMATICAL METHODS

I. A. Blaydа, N. Yu. Vasylieva, T. V. Vasylieva, L. I. Sliusarenko, O. I. Dzhambek

Odesa National Mechnykov University, Ukraine

The aim of the work was to optimize the process of bacterial desulfurization of energy coal, namely, to determine the influence of the component composition of the nutrient medium and the conditions of the process, which ensure the maximal development and activity of the aboriginal association of acidophilic chemolithotrophic bacteria and, as a consequence, the maximal index of sulfur decrease in coal in minimal time. We used the method of mathematical planning of the experiment adapted to the plan in Greek-Latin squares. The calculations in this approach are based on the analysis of variance (ANOVA). The formal planning of experiments has been carried out with four operating factors (nutrient medium components) at four levels (concentrations). The calculations were performed in Excel. The selection of operating factors and their combinations was made with the usage of unifactor ANOVA, correlation analysis and the method of principal components PCA. Researches were carried out in R 3.4.0 program and were founded on data of the preliminary evaluating experiments. Acidithiobacillus ferrooxidans Coal 17 aboriginal strain was used to obtain the most significant desulfurization effect. This strain was isolated from the investigated coal, studied and identified. The significance of the factor level for each nutrient medium component was analyzed using the Duncan’s multiple range test, the uniformity of the variances was examined with the Cochran’s test, and the significance of the factors was tested by the Fisher’s criterion. As a result, for the optimal nutrient medium the next combination of factors and their levels, which corresponds to the composition, g/dm³, was recommended: (NH₄)₂SO₄ — 0.15; K₂HPO₄ — 0.50; FeSO₄.7H₂O — 44.0; KCl — 0.10; MgSO₄·7H₂O — 0.10; Ca(NO₃)₂ — 0.10; yeast extract — 0.025% (vol.); strain A. ferrooxidans Coal 17 (titre 1·10⁸ CFU/ml) — 1.60% (vol.). This makes it possible to reduce the sulfur content in coal by 66.31% in a short period (seven days). This result could not be got before.

Key words: desulfurization, aboriginal association, acidophilic chemolithotrophic bacteria, plan in Greek-Latin squares, variance analysis.

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Details: Hits: 111

ISSN 2410-776X (Online)
ISSN 2410-7751 (Print)

6 2018

Biotechnologia Acta, V. 11, No 6, 2018
https://doi.org/10.15407/biotech11.06.047
Р. 47-54, Bibliography 25, English
Universal Decimal Classification: 57.023: 58.039

NANOSTRUCTURED FERRIC CITRATE EFFECT ON Chlorella vulgaris DEVELOPMENT

N. B. Golub¹, M. Tsvetkovych¹, I. I. Levtun¹, V. I. Maksyn²

¹National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute”
²National University of Life and Environmental Sciences of Ukraine, Kyiv

The aim of the research was to study the development of Chlorella vulgaris at culturing on the modified Gromov 6 medium with high concentrations of nanostructured ferric citrate and also its effect on photosynthesis pigments accumulation. It was demonstrated that the highest intracellular iron content (15 mg/g of dry mass) in the culture cells was typical with nanostructured ferric citrate content of 30 mg/dm3 of culture medium, the highest content of chlorophyll a — 23 mg/g of dry algae mass, b — 7.5 mg/g of dry mass, and carotenoids — 9.2 mg/g of dry mass was observed at nanostructured ferric citrate content of 20 mg/dm³. The use of nanostructured ferric citrate leaded to an increase in the chlorella biomass yield by 3 times with compared to standard technology. Simultaneously, intracellular iron content in cells increased significantly with the use of nanostructured ferric citrate, which increases their value as a nutritional supplement. In order to increase the biomass yield and intracellular iron content in cells, application of nanostructured ferric citrate is recommended.

Key words: chlorella cells, biomass, intracellular iron, chlorophylls, carotenoids, nanostructured ferric citrate.

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