- Details
- Hits: 1764
ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
Biotechnologia Acta, V. 11, No 3, 2018
https://doi.org/10.15407/biotech11.03.083
P. 83-88, Bibl. 33, Engl..
УДК:581.1631.811.98633.854.78581.198636.09
Makogonenko S. Yu., Baranov V. I., Ponomarenko S. P.
1Lviv National Ivan Franko University, Ukraine
2Interdepartmental scientific and technological center"Agrobiotech" of the National Academy of Sciences of Ukraine and the Ministry of Education and Science of Ukraine
The aim of study was to investigate the effect of new growth regulators “Stimpo” and “Regoplant”, produced by State Enterprise Interdepartmental Science and Technology Center ISTC "Agrobiotech", on the proline and free amino acids content, and lipid peroxidation reactions intensity in comperison to the action of gibberellic acid and “Treptolem” (a second-generation growth regulator) in 14 day sunflower sprouts. The plants were cultured on soil substrates made from the coal mine rock waste of the Central Concentrating Factory in the Chervonohrad mining region.
It was shown that the free amino acids and proline content increased, and the intensively of lipid peroxidation reactions (evaluated by the content of malonic dialdehyde) decreased. This indicated to the increase of plants resistance to unfavorable conditions of the coal mine rock under the action of the growth regulators studied.
One could assump that “Stimpo” and “Regoplant” are promising agents in phytochemical treatment of coal mine rock dumps.
Key words: Helianthus annuus L., "Stimpo", "Regoplant", "Treptolem", substrates of coal dumps, free amino acids, proline, lipid peroxidation reactions.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2018
References
1. Glukhov A. Z., Kharkhota A. I., Prokhorov S. I., Agurova I. V. Theoretical prefixes of population monitoring of phytoreculation of technogenic lands. Ecol. Noosphereol. 2010, 21, 3–4. (In Russian).
2. Baker A. J. M., McGrath S. P. C., Sidoli M. D., Reeves B. D. The possibility of in situ heavy metal decontamination of polluted soils using crops of metal accumulating plants. Resources, Conservation and Recycling. 1994, 11, 41–49. (In English).
3. Novitsky M. L. Granulometric composition of fine-grained sulfide rock and man-made substrates of mine dumps. Bulletin Nikitsky Experimental articles 87 Botan. Garden. Agroecology. 2011, 103, 85–89. (In Russian).
4. David E. Salt, Roger C. Prince, lngrid J. Pickering, llya Raskin. Mechanisms of cadmium mobility and accumulation in Indian mustard. Plant Physiol. 1995, 109, P. 1427–1433. (In English).
5. Smetanin V. I. Land recultivation: a review of technologies. Moscow: Ecol. Industrial. Rus. 2004, 45 p. (In Russian).
6. Lukina N. V., Chibrik Т. S., Glazyrina M. A., Filimonova E. I. Biological Recultivation and monitoring of disturbed land industry. Biological Systems. Yekaterinburg. 2014, 6 (2), 256 p. (In Russian).
7. Ratushniy V. M., Bondarenko A. M., Malakhovsky M. I. Determination of biological suitability of plants for biological reclamation of dumps of overburden. Zaporizhzhya: Bioindicat. Ecol. questions. 2011, 16 (1), 29–36. (In Ukrainian).
8. Baranov V. I., Gavrilyak M. Ya., Telegus Ya. V. Synthesis of amino acids, protein and nitrogencontaining compounds in rapeseed plants under conditions of growth on the substrate of the waste heap. Visnyk LNUVMB named after S. Z. Gzhytskyj. 2010, 12, No. 2 (4), 154–159. (In Ukrainian).
9. Baranov V., Beshley S., Telegus Ya. Some biochemical indices of adaptation of groundwater (Calamagrostis epigeios (L.) Roth) to the conditions of edaphotop of coal mines dumps. Visnyk Lviv University. 2012, 58, 292–299. (In Ukrainian).
10. Baranov V. I., Knysh I. M, Blida I. A., Vaschuk S. P., Gavrylyak S. М. The usual ocheret is a phytoreemedient of heavy metals in drainage ditches of waste heaps of coal mines. Studia biologica. 2012, 6 (1), 93–100. (In Ukrainian).
11. Gashchyshyn V. R., Patsula O. I., Terek O. I. Accumulation of heavy metals in Brassica napus L. and Helianthus annuus L. under the influence of zinc salts and growth regulator of “Treptolem”. Physiol. Plants and Genetics. 2014, 46 (4), 343–350. (In Ukrainian).
12. Gritsenko Z. M., Ponomarenko S. P., Kar penko V. P., Leontyuk I. B. Biologically active substances in crop production. Kyiv: “Nichlawa”. 2008, P. 179–191. (In Ukrainian).
13. Oliynyk О. O., Furman V. M., Solodka T. M., Vakulenchuk S. I. Study of the effectiveness of pre-seed treatment of seeds by stimulators of plant growth. Bulletin Natl. Univer. Water Management and Nature Management. 2013, 4 (64), 112–118. (In Ukrainian).
14. Sergeeva L. E., Bronnikova L. I., Tishchenko E. N. The content of free proline as an indicator of the vitality of the Nicotiana tabacum L. Cell culture under stress. Biotechnology. 2011, 4 (4), 87–94. (In Russian).
15. Babayants A. V., Gritsenko Z. M., Ponomarenko S. P. Biostimulators (growth regulators) of plants. Kyiv: ISTC Agrobiotech. 2014, Р. 3–15. (In Ukrainian).
16. Ponomarenko S. P. Plant growth regulators. Bulletin Institute Bioorg. Chem. 1976, 319 p. (In Ukrainian).
17. Ponomarenko S. P., Terek O. I., Hrytsaenko Z. M. Bioregulation of plant growth and development. Bioregul. Microb. plant systems. Kyiv: Nichlava. 2010, P. 251–291. (In Ukrainian).
18. Pida S. V., Triguba O. V., Grigoruk I. P. Effect of bacterial preparations and plant growth regulators on the photosynthetic apparatus of white lupine (Lupinus albus L.). Bioresources and nature use. 2014, 6 (1, 2), 12–18. (In Ukrainian).
19. GOST 12038-84. Methods of determining the germination and energy of germination. Seeds of agricultural crops. Moskva: Publishing of Standards. 1984, 57 p. (In Russian).
20. Pollard A. J. Metal hyperaccumulation: a model system for coevolutionary studies. New Phytol. 2000, 146, 179–181.
21. Makogonenko S. Yu., Baranov V. I., Kar pinets L. I., Ponomarenko S. P., Terek O. I. Influence of gibberellin, Stippo and Regaplant on growth and content of pigment photosynthesis and protein of Hellianthus annuus L. on man-made substrates. Botany (researches): Collection of scientific works. Institute Experiment. Botan. Kuprevich of the National Academy of Sciences of Belarus. Issue 46. 2017, 295–303 (In Russian).
22. Pirog О. V. Increase virus resistance of plants of lupine yellow under the action of microbial drugs and physiologically active substances. Agricultural Microbiology. 2016, 24, 59–63. (In Ukrainian).
23. Pochinok H. N. Methods of Biochemical Analysis of Plants. Kyiv: Sciences. оpinion. 1976, 85 p. (In Russian).
24. Beatles L.S., Waldren P., Teare D. Rapid determination of free proline for water-stress studies. Plant Soil. 1973, 39, 205–207.
25. Heath R. L., Packer L. Photoperoxidation in isolated chloroplasts. Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys. 1968, 125, 189–198. https://doi.org/10.1016/0003-9861(68)90654-1
26. Baranov V., Beshley S., Telegus Ya. Changes in the content of sulfur, free amino acids and protein in rapeseed plants, fertilized with encapsulated fertilizers on the substrates of the waste dump of coal mines. Studia biol. 2010, 4 (1), 53–62. (In Ukrainian).
27. Маlenka U., Kobyletska M., Terek O. Effect of salicylic acid on the content of free amino acids and proline on wheat and maize plants under drought conditions. Studia biol. 2014, 8 (2), 123–132. (In Ukrainian).
28. Grebinsky S. Biochemistry of plants. Lviv: Higher school. Publishing house at Lviv. Unte. 1967, 272 p. (In Russian).
- Details
- Hits: 821
ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
"Biotechnologia Acta" V. 11, No 3, 2018
https://doi.org/10.15407/biotech11.03.078
Р. 78-82, Bibliography 7, English
Universal Decimal Classification: 628.315.3.004
ISOLATION OF PURE CULTURES IRON- AND MANGANESE-OXIDIZING BACTERIA FROM RAPID FILTERS
O. V. Kravchenko, O. S. Panchenko
Scientific, Research, Design and Technology Institute of Municipal Economy, State Enterprise, Kyiv, Ukraine
The aim of the research was the isolation from drinking water the pure cultures of iron- and manganese-oxidizing microorganisms with further assessment of their efficacy to remove these contaminants on rapid filters. To assess the effectiveness selected strains were grown on the solid nutrient medium; the suspension was prepared and was treated to zeolite loading. Ten pure cultures of iron- and manganese-oxidizing bacteria were isolated and identified as 6 genuses: Siderocapsa, Leptothrix, Sphaerotillus, Galionella, Metallogenium, Hyphomicrobium. Comparison the efficiency of genuses Leptothrix, Sphaerotillus, Metallogenium has shown that under conditions of these experiments Leptothrix more effectively removed iron and manganese at low concentrations in model solution.
Key words: iron- and manganese-oxidizing microorganisms, rapid filters, zeolite loading.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2018
References
1. Farkas А., Dragan-Bularda М., Muntean V., Ciataras D., Tigan S. Microbial activity in drinking water-associated biofilms. Cent. Eur. J. Biol. 2013, 8 (2), 201–214. https://doi.org/10.2478/s11535-013-0126-0
2. Dubinina G. A., Sorokina A. Y., Mysyakin A. E., Grabovich M. Y., Eprintsev A. T., Bukreeva V. Y. Modelling and optimization of processes for removal of dissolved heavy-metal compounds from drinking water by microbiological methods. Wat. Resources. 2012, 39 (4), 398–404. doi: 10.1134/s00978078 12030037.
3. De Vet W. W. J. M., Dinkla I. J. T., Rietveld L. C., van Loosdrecht M. C. M. Biological iron oxidation by Gallionella spp. in drinking water production under fully aerated conditions. Wat. Research. 2011, 45 (17), 5389–5398. doi: 10.1016/j.watres. 2011.07.028.
4. Qin S., Ma F., Huang P., Yang J. Fe (II) and Mn (II) removal from drilled well water: A case study from a biological treatment unit in Harbin. Desalination. 2009, 245 (1–3), 183–193. https://doi.org/10.1016/j.desal.2008.04.048
5. Zakharova Yu. R., Parfenova V. V. A method for cultivation of microorganisms oxidizing iron and manganese in bottom sediments of Lake Baikal. Biol. Bull. 2007, 34 (3), 236–241. https://doi.org/10.1134/S1062359007030041
6. Zaharova Ju. R. Microorganisms oxidizing iron and manganese in bottom sediments of Lake Baikal (Doctoral dissertation). Available from Dissercat database. 2007.
7. Kvartenko O. M. Use of anchored microflora to clear groundwater with high iron content (Doctoral dissertation). Available from Dissercat database. 1997.
- Details
- Hits: 936
ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
"Biotechnologia Acta" V. 11, No 3, 2018
https://doi.org/10.15407/biotech11.03.069
Р. 69-77, Bibliography 13, English
Universal Decimal Classification: 582.284:543.061
GANODERMA SPECIES EXTRACTS: ANTIOXIDANT ACTIVITY AND CHROMATOGRAPHY
V. Raks 1, 2, M. Öztürk 2, O. Vasylchenko 3, M. Raks 4
1 Taras Shevchenko National University of Kyiv, Ukraine
2 Mugla Sitki Kocman University, Turkey
3 National Aviation University, Kyiv, Ukraine
4 National University of Food Technologies, Kyiv, Ukraine
Research aimed to isolate biologically active compounds from mushrooms fruiting bodies of Ganoderma lucidum, Ganoderma adspersum and Ganoderma applanatum and estimate their antioxidant activities. Variety of sample preparation techniques were used to isolate biologically active compounds: solid-liquid, ultrasonic extraction, Soxhlet extractor extractions. Antioxidant properties were estimated with spectrophotometricaly measuring free radical scavenging activity. High performance liquid chromatography was applied to get fingerprints of isolated extracts. In β-carotene-linoleic acid assay, methanol extracts demonstrated mainly the best antioxidant activities: optimum values of half maximal inhibitory concentration (IC50) for G. applanatum and G. adspersum were 8.25 ± 0.88 µg/ml and 1.70 ± 1.13 µg/ml respectively. However, petroleum ether and chloroform extracts of G. lucidum demonstrated the highler antioxidant activity with an IC50 about 33.66 ± 3.69 µg/ml. Chromatograms of dry components of acetone and methanol extracts of G. lucidum were recorded. The main outcome of such chromatograms is the possibility to compare presence active components in various mushrooms species without usage of expensive standards.
Key words: Ganoderma species mushrooms, antioxidant activity, high performance liquid chromatography.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2018
References
1. Yahia E. M., Guti?rrez-Orozco F., Moreno-P?rez M. A. Identification of phenolic compounds by liquid chromatography-mass spectrometry in seventeen species of wild mushrooms in Central Mexico and determination of their antioxidant activity and bioactive compounds. Food Chem. 2017, 226, 14–22. https://doi.org/10.1016/j.foodchem.2017.01.044
2. Ma G., Yang W., Zhao L., Pei F., Fang D., Hu Q. A Critical Review on the Health Promoting Effects of Mushrooms Nutraceuticals. Food Sci. 2018. Human Wellness. In press, accepted manuscript.
3. Anita Klaus, Maja Kozarski, Jovana Vunduk, Predrag Petrovi?, Miomir Nik?i?. Antibacterial and antifungal potential of wild basidiomycete mushroom Ganoderma applanatum. Lek. Sirovine. 2017, 36, 37–46.
4. Elkhateeb W. A., Zaghlol G. M., El-Garawani I. M., Ahmed E. F., Rateb M. E., Abdel Moneim A. E. Ganoderma applanatum secondary metabolites induced apoptosis through different pathways: In vivo and in vitro anticancer studies. Biomed. Pharm. 2018, 101, 264–277. https://doi.org/10.1016/j.biopha.2018.02.058
5. Komura D. L., Carbonero E. R., Gracher A. H. P., Baggio C. H., Freitas C. S., Marcon R., Santos A. R. S., Gorin P. A. J., Iacomini M. Structure of Agaricus spp. fucogalactans and their antiinflammatory and antinociceptive properties. Bioresource Technol. 2010, 101, 6192–6199. https://doi.org/10.1016/j.biortech.2010.01.142
6. Cheng C.-R., Yue Q.-X., Wu Z.-Y., Song X.-Y., Tao S.-J., Wu X.-H., Xu P.-P., Liu X., Guan S.-H., Guo D.-A. Cytotoxic triterpenoids from Ganoderma lucidum. Molecules. 2018, 23, 649. a href="https:/doi.org/10.3390/molecules23030649%20101,%20264–277." " ">"nbsp;https://doi.org/10.3390/molecules23030649 101, 264–277.
7. Ferreira A., Proenca C., Serralheiro M. L. M., Araujo M. E. M. The in vitro screening for acetylcholinesterase inhibition and antioxi dant activity of medicinal plants from Portugal. J. Ethno Pharm. 2006, 108, 31–37, https://doi.org/10.1016/j.jep.2006.04.010.
8. Asakawa Y., Nagashima F., Hashimoto T., Toyo ta M., Ludwiczuk A., Komala I., Ito T., Yagi Y. Pungent and bitter, cytotoxic and antiviral terpenoids from some bryophytes and inedible fungi. Nat. Prod. Commun. 2014, 9, 409–417.
9. Sakamoto S., Kikkawa N., Kohno T., Shimizu K., Tanaka H., Morimoto S. Immuno chro matographic strip assay for detection of bioactive Ganoderma triter penoid, ganoderic acid A in Ganoderma lingzhi, Fitoterapia. 2016. doi: 10.1016/j.fitote. 2016.08.01.
10. Tel-?ayan G. l., ?zt?rk M., Duru M. E., Reh man M. U., Adhikari A., T?rko?lu A., Choudhary M. I. Phytochemical investigation, antioxidant and anticholinesterase activities of Ganoderma adspersum. Industr. Crops Prod. 2015, 76, 749–754. https://doi.org/10.1016/j.indcrop.2015.07.042
11. Blois M. S. Antioxidant determinations by the use of a stable free radical. Nature. 1958, 181, 1199–1200. https://doi.org/10.1038/1811199a0
12. Re R., Pellegrini N., Proteggente A., Pannala A., Yang М., Rice-Evans С. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Rad. Bio. Med. 1999, 26, 1231–1237.
13. Apak R., G??l? K., ?zy?rek M., Karademir S. E. Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, Using their cupric ion reducing capability in the presence of neocuproine CUPRAC Method. J. Agr. Food Chem. 2004, 52, 7970–7981.
- Details
- Hits: 880
ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
"Biotechnologia Acta" V. 11, No 3, 2018
https://doi.org/10.15407/biotech11.03.056
Р. 56-68, Bibliography 34, English
Universal Decimal Classification: 635.8:577.19
Vlasenko E. N., Kuznetsova O. V.
Ukrainian State University of Chemical Technology, Dnipro, Ukraine
The purpose of the study was to analyze possible ways and intensity of synthesis of volatile flavor compounds by Pleurotus ostreatus (Jacq.:Fr.) Kumm. mushrooms in the process of intensive cultivation on sunflower husk and barley straw with the addition of vegetable oils (sunflower and corn) as a potential source of unsaturated fatty acids. Methods of sensory profile analysis and ultraviolet spectroscopy were used. Sensory profile analysis of dried samples of fruit bodies showed an increase in the intensity of mushroom, meat and grassy notes of flavor on substrates with the addition of vegetable oils in a concentration of 1% and 5% of the weight of the substrate. For the strain IBK-551 marked increase in the intensity of sweet and floral attributes of the aroma on both substrates with the addition of corn oil. UV spectroscopy of hexane extracts of dried samples of fruit bodies revealed maxima of light absorption in the range of 200–210 nm and 260–300 nm. There was a difference in intensity of light absorption of samples of different strains cultivated on substrates with the addition of vegetable oils.
Key words: Pleurotus ostreatus, volatile aroma compounds, sunflower oil, corn oil, sensory profile analysis, UV spectroscopy.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2018
References
1. Kalac P. A review of chemical composition and nutritional value of wild-growing and cultivated mushrooms. J. Sci. Food Agric. 2013, 93, 209–218. https://doi.org/10.1002/jsfa.5960
2. Fraatz M. A., Zorn H. Fungal flavours. Industrial applications. The mycota (A comprehensive treatise on fungi as experimental systems for basic and applied research). Hofrichter M. (eds). Springer, Berlin, Heidelberg. 2011, 10, 249–268. https://doi.org/10.1007/978-3-642-11458-8_12
3. Combet E., Henderson J., Eastwood D. C., Burton K. S. Eight-carbon volatiles in mushrooms and fungi: properties, analysis, and biosynthesis. Mycoscience. 2006, 47, 317–326. https://doi.org/10.1007/S10267-006-0318-4
4. Cho I. H., Namgung H.-J., Choi H.-K., Kim Y.-S. Volatiles and key odorants in the pileus and stipe of pine-mushroom (Tricholoma matsutake Sing.). Food Chem. 2008, 106, 71–76. https://doi.org/10.1016/j.foodchem.2007.05.047.
5. Splivallo R., Ottonello S., Mello A., Karlovsky P. Truffle volatiles: from chemical ecology to aroma biosynthesis. New Phytologist. 2011, 189 (3), 688–699. https://doi.org/ 10.1111/j.1469-8137.2010.03523.x.
6. Zeppa S., Gioacchini A. M., Guidi C., Guescini M., Pierleoni R., Zambonelli A., Stocchi V. Determination of specific volatile organic compounds synthesised during Tuber borchii fruit body development by solid-phase microextraction and gas chromatography-mass spectrometry. Rapid Commun. Mass Spectrom. 2004, 18, 199–205.https://doi.org/10.1002/rcm.1313
7. Wu С.-M., Wan Z. Volatile compounds in fresh and processed shiitake mushrooms (Lentinus edodes Sing.). Food Sci. Technol. Res. 2000, 6 (3), 166–170. https://doi.org/10.3136/fstr.6.166
8. Feussner I. Oxylipins in fungi. FEBS J. 2011, 278, 1047–1063. doi: 10.1111/ j.1742-4658.2011.08027.x.
9. Senger T., Wichard T., Kunze S., Gobel C., Lerchl J., Pohnert G., Feussner I. A multi functional lipoxygenase with fatty acid hydroperoxide cleaving activity from the moss Physcomitrella patens. J. Biol.Chem. 2005, 280 (9), 7588–7596. https://doi.org/10.1074/jbc.M411738200
10. Zheljazkov V. D., Vick B. A., Ebelhar M. W., Buehring N., Baldwin B. S., Astatkie T., Miller J. F. Yield, oil content, and composition of sunflower grown at multiple locations in Mississippi. Agr. J. 2008, 100 (3), 635–642. doi: 10.2134/ agronj2007. 0253.
11. Tymchuk D. S., Muzhylko V. V., Demchenko D. A. Content and fatty acid composition of oil in grain of corn endosperm mutants. Visnyk Harkivskoho natsionalnoho ahrarnoho universytetu, seriia biolohiia. 2017, 2 (41), 85–91. (In Ukrainian).
12. DSTU 4492:2005 Sunflower oil. Specifications. Existing from 01. 01. 2007. Kyiv: Derzhspozhyvstandart Ukrainy. 2006, 22 p. (In Ukrainian).
13. DSTU GOST 8808:2003 Corn oil. Specifications. Existing from 01. 01. 2004. Kyiv: Derzhspozhyvstandart Ukrainy. 2003, 12 p. (In Ukrainian).
14. Bisko N. A., Lomberg M. L., Mytropolska N. Yu., Mykchaylova O. B. The IBK Mushroom Culture Collection. Kyiv: Kholodny Institute of Botany of the National Academy of Sciences of Ukraine: Alterpres. 2016, 120 p.
15. Buhalo A. S., Bis’ko N. A., Solomko E. F., Poedinok N. L., Mihajlova O. B. The cultivation of edible and medicinal mushrooms. Кyiv: Chornobylinterinform. 2004, 127 p. (In Russian).
16. Vlasenko K. M., Kuznetcova O. V. The use of sensory analysis in biotechnology of the cultivation of macromycetes. Visn. Dnipropetr. Univ. Ser. Biol. Ekol. 2016, 24 (2), 347–352. https://doi.org/10.15421/011645
17. Atramentova L. O., Utevs’ka O. M. Statistics for biologists. Harkіv: Vydavnytstvo “NTMT”. 2014, 331 p. (In Ukrainian).
18. Tasaki Y., Toyama S., Kuribayashi T., Joh T. Molecular characterization of a lipoxygenase from the basidiomycetes mushroom Pleurotus ostreatus. Biosci. Biotechnol. Biochem. 2013, 77 (1), 38–45. https://doi.org/10.1271/bbb.120484
19. Gardner H. W. Recent investigations into the lipoxygenase pathway of plants. Biochim. Biophys. Acta. 1991, 1084, 221–239. https://doi.org/10.1016/0005-2760(91)90063-N
20. Reis F. S., Barros L., Martins A., Ferreira I. Chemical composition and nutritional value of the most widely appreciated cultivated mushrooms: an inter-species comparative study. Food Chem. Toxicol. 2012, 50 (2), 191–197.https://doi.org/10.1016/j.fct.2011.10.056
21. Wurzenberger M., Grosch W. The formation of 1-octen-3-ol from the 10-hydroperoxide isomer of linoleic acid by a hydroperoxide lyase in mushrooms (Psalliota bispora). Biochim. Biophys. Acta. 1984, 794 (1), 25–30. doi: 10.1016/0005 -2760(84)90293-5.
22. Akakabe Y., Matsui K., Kajiwara T. Stereochemical correlation between 10-hydro peroxyoctadecadienoic acid and 1-octen-3-ol in Lentinula edodes and Tricholoma matsutake mushrooms. Biosci. Biotechnol. Biochem. 2005, 69 (8), 1539–1544. https://doi.org/10.1271/bbb.69.1539
23. Assaf S., Hadar Y., Dosoretz C. G. 1-Octen-3-ol and 13-hydroperoxylinoleate are products of distinct pathways in the oxidative breakdown of linoleic acid by Pleurotus pulmonarius. Enz. Microb. Technol. 1997, 21 (7), 484–490. doi: 10.1016/ S0141-0229(97)00019-7.
24. Cheng A.-X., Lou Y.-G., Mao Y.-B., Lu S., Wang L.-J., Chen X.-Y. Plant terpenoids: biosynthesis and ecological functions. J. Integr. Plant Biol. 2007, 49 (2), 179–186. doi: 10.1111/j.1672-9072.2006.00395.x.
25. Heldt G.-V. Plant biochemistry. Moskva: Binom. Laboratoriya znaniy. 2011, 471 p. (In Russian).
26. Dudareva N., Klempien A., Muhlemann J. K., Kaplan I. Biosynthesis, function and metabolic engineering of plant volatile organic compounds. New Phytologist. 2013, 198, 16–32.https://doi.org/10.1111/nph.12145
27. Lee W.-J., Banavara D. S., Hughes J. E., Christiansen J. K., Steele J. L., Broadbent J. R., Rankin S. A. Role of cystathionine ?-lyase in catabolism of amino acids to sulfur volatiles by genetic variants of Lactobacillus helveticus CNRZ 32. Appl. Environm. Microbiol. 2007, 73 (9), 3034–3039. doi: 10.1128/ AEM.02290-06.
28. Liu Y., Lei X.-Y., Chen L.-F., Bian Y.-B., Yang H., Ibrahim S. A., Huang W. A novel cysteine desulfurase influencing organosulfur compounds in Lentinula edodes. Sci. Rep. 2015, 5, 10047. https://doi.org/10.1038/srep10047
29. Yasumoto K., Iwami K., Mitsuda H. Enzy me-catalized evolution of lenthionine from lentinic acid. Agricult. Biol. Chem. 1971, 35 (13), 2070–2080. doi: 10.1080 /00021369.1971.10860188.
30. Hu C., Zou Y., Zhao W. Effect of soybean oil on the production of mycelial biomass and pleuromutilin in the shake-flask culture of Pleurotus mutilis. World J. Microbiol. Biotechnol. 2009, 25, 1705–1711. https://doi.org/10.1007/s11274-009-0064-9
31. Kalyoncu I. H., KaSik G., Ozcan M., Ozturk C. Effects of sesame and bitter almond seed oils on mycelium growth of Agaricus bisporus (Lange) Sing. Grasas y Aceites. 1999, 50 (5), 392–394.
32. Rodina T. G. Sensory analysis of food products. Moskva: Akademiya. 2004, 208 p. (In Russian).
33. Sil’verstejn R., Bassler G., Morril T. Spectrometric identification of organic compounds. Moskva: Mir. 1977, 590 p. (In Russian).
34. Vlasenko K. M., Kuznetcova O. V., Stepnevs’ka Ja. V. Influence of mineral substances on the synthesis of volatile organic compounds by Pleurotus ostreatus in the process of solid phase cultivation. Regul. Mech. Biosyst. 2017, 8 (4), 489–496. https://doi.org/10.15421/021775.
- Details
- Hits: 1399
ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
"Biotechnologia Acta" V. 11, No 3, 2018
https://doi.org/10.15407/biotech11.03.047
Р. 47-55, Bibliography 27, English
Universal Decimal Classification: 631.81.033:574
APPLICATION OF BIOFILMS IN REMOVAL OF HEAVY METALS FROM WASTEWATER IN STATIC CONDITION
Ogbuagu Dike Henry 1, Nwachukwu Ikenna Ndubuisi 2, Ejike Onyinye Joy 1
1 Department of Environmental Technology, Federal University of Technology, Owerri, Nigeria
2 Department of Microbiology, Federal University of Technology, Owerri, Nigeria
The aim of the research was to utilize biofilms as a model in ecotoxicology to remove selected heavy metals (Cd, Cu, Cr, Zn and Pb) from wastewater in a static condition. Biofilms were grown in three graded concentrations of the metal leachates (0.625, 0.417 and 0.250 %), harvested after 1, 2 and 3 weeks and analyzed for heavy metals. Mean accumulations peaked on Day 21, and of Cd ranged from 0.000 to 0.040 (mean = 0.00837 ± 0.002), Cu from 0.000 to 0.212 (meam = 0.03929 ± 0.012), Cr from 0.000 to 0.500 (mean = 0.05821 ± 0.021), Zn from 0.000 to 1.456 (mean = 0.31833 ± 0.109) and Pb from 0.000 to 0.099 (mean = 0.02129 ± 0.006) mg/g in resultant biofilm formations. Accumulation of the metals increased significantly with time [F(205.59) > Fcrit(3.95)] at the 95% confidence interval. Those of Pb was significantly higher in the 0.625% leachate mixture than control (Sig F = 0.034) at P < 0.05, even as those of Cd and Cu were slightly higher in the concentrations than control. Biofilm model removed small amounts of metals from wastewater stream in static condition.
Key words: heavy metals, biofilms, bioaccumulation, wastewater, static condition.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2018
References
1. Costa A. D., Carlis A., Pereira F. Bioaccumulation of copper, zinc, cadmium and lead by Bacillus Spp., Bacillus cereus, Bacillus sphaericus and Bacillus subtilis. Braz. J. Microbiol. 2006, 32 (1), 1–5. https://doi.org/10.1590/S1517-83822001000100001
2. Barakat M. A. New Trends in Removing Heavy Metals from Industrial Waste water. Arab. J. Chem. 2011, 4, 361–377. https://doi.org/10.1016/j.arabjc.2010.07.019
3. Ogbuagu D. H., Okoli C. G., Emereibeole E. I., Anyanwu I. C., Onuoha O., Ubah N. O., Ndugbu C. O., Okoroama O. N., Okafor A., Ewa E., Ossai R., Ukah F. Trace metals accumulation in biofilms of the upper and middle reaches of Otamiri River in Owerri, Nigeria. J. Biodiver. Environm. Sci. (JBES). 2011, 1 (3), 19–26.
4. Babel S., Kurniawan T. A. Low-cost absorbent for heavy metals uptake from contaminated water: a review. J. Hazard. Mater. 2003, 97 (1), 219–243. https://doi.org/10.1016/S0304-3894(02)00263-7
5. Perpetuo E. A. Engineering bacteria for bioremediation. In carpi A. (Ed.) Progress in molecular and environmental Bioengineering from analysis and modeling to technology application. Rijika: Intech. 2011, 605–632.
6. Mansour S. A., Sidky M. M. Ecotoxicological studies: heavy metals contaminating water and fish from Fayum Governorate, Egypt. Food Chem. 2002, 78 (1), 15–22. https://doi.org/10.1016/S0308-8146(01)00197-2
7. Fomina M., Gadd G. M. Biosorption: current perspectives on concept, definition and application. Bioresource Technol. 2014, 160, 3–14. https://doi.org/10.1016/j.biortech.2013.12.102
8. Volesky B., Holan Z. K. Biosorption of heavy metals. Biotechnol. Progr. 1995, 11 (3), 235–250. https://doi.org/10.1021/bp00033a001
9. Eccles H. Treatment of metal contamination waste. Why select a biological process? Trends Biotechnol. 1999, 17, 462–465. https://doi.org/10.1016/S0167-7799(99)01381-5
10. Srivastava S., Agrawal S., Mondal M. A review on progress of heavy metal removal using adsorption of microbial and plant origin. Environm. Sci. Pollut. Res. 2013, 22 (20), 15386–15415. https://doi.org/10.1007/511356-5278-9PMID26313592.
11. Costerton J. W., Lewandowski Z., De Beer D., Caldwell D., Korber D., Jamese G. Minireview: biofilms, the customized microniche. J. Bacteriol. 1994, 176, 2137–2142. https://doi.org/10.1128/jb.176.8.2137-2142.1994
12. Wimpenny J. Heterogeneity in biofilms. FEMS Microbial Rev. 2000, 24, 661–667. https://doi.org/10.1111/j.1574-6976.2000.tb00565.x
13. Deibel V., Schoeni J. Biofilms: Forming a defense strategy for the food plant. Food Safety Magazine, De. 2003, 2002/Jan. 2003 edition.
14. Sutherland I. W. The biofilm matrixan immobilized but dynamic microbial environment. Trend Microbial. 2001, 9, 222–227. https://doi.org/10.1016/S0966-842X(01)02012-1
15. Flemming H-C., Nue T. R.,Wozniak D. J. The EPS matrix: the “house of biofilm cell”. J. Bacteriol. 2007, 189 (22), 7945–7947. https://doi.org/10.1128/JB.00858-07
16. Gupta V. K., Nayak A., Aganoval S. Biosorbents for remediation of heavy metals: current status and their future prospects. Environm. Engineer. Res. 2015, 20 (1), 1–18.
17. Meylan S., Sigg L., Behra R. Metal accumulation in algal biofilms. Eawag: Swiss Federal Institute of Aquatic Science and Technology. 2006, 60e, 19–21.
18. Aryal M. Removal and recovery of Nickel ions from Aqueous solution using bacillus Sphaericcus Biomass. Int. J. Environm. Res. 2015, 9 (4), 1147–1156.
19. IIyina A., Castillo S. M. I., Villarreal S. J. A., Ramirez E. G., Candelas R. J. Isolation of soil bacteria for bioremediation of hydrocarbon contamination. Vestnik Mosk. un-ta. Ser. 2. Khimiya. 2003, 44 (1).
20. Zhang W. Removal of hexavalent chromium from waste water using magmetotactic bacteria. Separ. Purific. Technol. 2014, 136, 10–17. https://doi.org/10.1016/j.seppur.2014.07.054
21. Chipasa K. B. Accumulation and fate of selected heavy metals in a biological waste water treatment system. Waste Management. 2003, 23 (2), 135–143. PMID: 12623088. https://doi.org/10.1016/S0956-053X(02)00065-X
22. Karvalas M., Katsogiannis A., Samara C. Occurrence and fate of heavy metals in the waste water treatment process. Chemosphere. 2003, 53 (10), 120–10. PMID: 14550351.
23. Doering M., Uehlinger U. Biofilms in the Tagliamento. Eawag: Swiss Fed. Inst. Aquatic Sci. Technol. 2006, 60e, 11–13.
24. Azizi S., Valipour A., Sithebe T. Evaluation of different waste water treatment processes and development of a Modified Attached Growth Bioreactor as a decentralized approach for small communities. Sci. Word J. 2013, 1–8. Article ID 156870. https://doi.org/10.1155/2013/156870
25. United Nations Environmental Programme Global Environment Monitoring System (UNEP GEMS)/Water Programme. Water quality for ecosystem and human health. UNEP-GEM System/Water Programme, Burlington, Ontario. 2006, 132 p.
26. Huang Y., Zhang D., Xu Z., Yuan S., Li Y., Wang L. Effect of overlying water pH, dissolved oxygen and temperature on heavy metal release from river sediments under laboratory conditions. Arch. Environm. Protect. 2017, 43 (2), 28–36. https://doi.org/10.1515/aep-2017-0014
27. Piccirillo C., Pereira S. Bacteria immobilization on hydroxyapatite surface for heavy metals. J. Environm. Manag. 2013, 121, 187–195. https://doi.org/10.1016/j.jenvman.2013.02.036.