1_2010(en)

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ISSN 1995-5537

"Biotechnology" journal Vol. 3, No. 1, 2010
Р. 9-17, Bibliography 63, Ukranian.
Universal Decimal classification: 575.222.7:581.1

LETTUCE (Lactuca sativa L.) AS AN OBJECT OF BIOTECHNOLOGY AND PRODUCER OF RECOMBINANT PROTEINS

N. А. Matvieieva

Institute of Cell Biology and Genetic Engineering of National Academy of Sciences of Ukraine, Kyiv

In the review the basic achievements in biotechnology of lettuce are cited. Such areas as lettuce cultivation іn vitro, development of protoplast isolation technique, somatic hybridization, regeneration of plants are considered. Achievements in the area of genetic transformation of lettuce and production of the resistant to biotic and abiotic factors transgenic plants and the plants – producers of recombinant proteins are described.

Key words: Lactuca sativa, biotechnology, somatic hybridization, genetic transformation, recombinant proteins.

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ISSN 1995-5537

"Biotechnology" journal Vol. 3, No. 1, 2010
Р. 18-26, Bibliography 62, Ukranian.
Universal Decimal classification: 546.26.043

FULLERENES С₆₀ - BIOLOGICALLY ACTIVE MOLECULES
І. Physicochemical properties and bioavailability

O. P. Matyshevska S. V. Prylutska I. I. Grynyuk

National Taras Shevchenko University, Kyiv

Analysis of the literature data concerning structure of a new class of nanocompounds – fullerenes C₆₀, their physical and chemical properties, and ways of introduction into biological systems, toxicity and perspectives of using as biologically active compounds is carried out.

Key words: fullerene С₆₀, С₆₀ watersoluble derivatives, ROS production, bioavailability.

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ISSN 1995-5537

"Biotechnology" journal Vol. 3, No. 1, 2010
Р. 27-34, Bibliography 29, Ukranian.
Universal Decimal classification: 577.151.6

PURIFICATION AND BIOCHEMICAL CHARACTERIZATION OF FIBRINOGENASE FROMVENOM OF Echis multisquamatis

V. O. Chernyshenko, M. P. Myasnikova, Т. М. Platonova, E. V. Lougovskoi,E. M. Makogonenko

National Taras Shevchenko University, Kyiv
Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv

Proteinase that demonstrated fibrinogenase activity from the venom of Echis multisquamatis was purified. Molecular weight of enzyme determined by SDS PAGE was 35 ± 1 kDа. Inhibitory assay showed that fibrinogenase belonged to the serine type of proteinases. It hydrolyzed X-X-R- pNa derivatives of tripeptides and splitted Bβ-chain of fibrinogen. Molecular weight of removed peptide of Bβ-chain allowed to suppose that fibrinogenase splitted ВβR23-E24 peptide bound. The K_m value of this reaction was 8 µM.

Key words: β-fibrinogenase, fibrinogen, venom of Echis multisquamatis.

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ISSN 1995-5537

"Biotechnology" journal Vol. 3, No. 1, 2010
Р. 35-40, Bibliography 14, Ukranian.
Universal Decimal classification: 577.151.083:615.917

FIBRINO(GENO)LYTIC ENZYME FROM Agkistrodon blomhoffii ussuriensis SNAKE VENOM: ISOLATION, PARTIAL CHARACTERIZATION AND INFLUENCE ON PLATELETS AGGREGATION

V. L. Karbovskiy, T. M. Platonova, O. V. Gornitska, G. L. Volkov

National Taras Shevchenko University, Kyiv
Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv

Fibrino(geno)lytic enzyme «Blomulyse» from Agkistrodon blomhoffii ussuriensis venom has been isolated in three chromatography steps. The purified enzyme is a typical single-chain metalloproteinase with molecular weight of approximately 30 kDa. Obtained enzyme hydrolyzed with high-efficiency the Aα-chain of fibrinogen followed by the Bβ-chain and showed no effect on the γ-chain.

Using different model systems in vitro we indicated that fibrolyse has no effect on the platelets aggregation stimulated by collagen, but on the other hand it inhibited ADP- and adrenalin-induced platelet aggregation.

Key words: fibrino(geno)lytic enzyme, snake venom, Agkistrodon, fibrinogen, platelets.

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ISSN 1995-5537

"Biotechnology" journal Vol. 3, No. 1, 2010
Р. 41-45, Bibliography 8, Russian.
Universal Decimal classification: 57.085.23:615.361.018.5.013.8

USE OF LOW TEMPERATURES FOR CREATION OF VASCULAR SCAFFOLDS

D. V. Byzov, O. P. Synchikova, I. P. Mikhaylova, S. M. Dergun, B. P. Sandomirskiy

Institute for Problems of Cryobiology and Cryomedicine of National Academy of Sciences of Ukraine, Kharkiv
National Technical University «Kharkiv Polytechnical Institute «NTU KhPI»

We investigated the effect of low temperatures on pig.s arterial vessels at creation of acellular xenogeneic vascular scaffolds. It was observed partial decellularization of endothelium layer, structure changing of connective tissue`s fibers and preservation of mechanical properties of vessels in a group of frozen-thawed arteries.

Key words: vascular grafts, scaffolds, bioengineering, decellularization.