SSN 2410-7751 (Print)
ISSN 2410-776X (Online)
License CC-BY.

Biotechnologia Acta V. 19, No. 5 2026
P, 76-85, Bibliography 25, Engl.
UDC 577.1:544.77:582.683.2
doi:

Full text: (PDF, in English)

COMPARATIVE STUDY OF GOLD NANOPARTICLES FORMATION BY EXTRACTS FROM TRANSGENIC AND NONTRANSFORMED Bidens pilosa L. ROOTS

N.A. MATVIEIEVA¹ (https://orcid.org/0000-0002-4877-5222)
V. P. DUPLIJ¹ (https://orcid.org/0000-0002-7479-7257)
T. A. BOHDANOVYCH¹ (https://orcid.org/0000-0002-1834-523X)
D. O. KLYMCHUK² (https://orcid.org/0000-0002-7076-8213)1

¹ Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine, Kyiv
² M. Kholodny Institute of Botany of the National Academy of Sciences of Ukraine, Kyiv

Aim. The study was aimed to compare the process of gold nanoparticles (AuNP) formation using extracts from transgenic and nontransformed roots of Bidens pilosa L., taking into account potential changes in the metabolism of transformed root cells following genetic transformation.

Methods. Transgenic roots and Bidens pilosa L. plants from the collection of the Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine were used. The flavonoid content and reducing activity of the ethanol extracts were determined, and the extracts were then used to synthesize AuNPs with 0.07% HAuCl₄. Nanoparticles were characterized spectrophotometrically over the range ƛ= 300–700 nm (SPR) and by transmission electron microscopy (TEM); the size distribution of nanoparticles was calculated from measurements of 200 nanoparticles in each variant (ImageJ).

Results. Extracts from transgenic roots showed greater reducing activity than extracts from control roots, despite the same concentration of flavonoids in the standardized extracts. The addition of extracts from both transgenic and control roots to the HAuCl₄ solution resulted in a characteristic change in the solution’s color. The presence of nanoparticles was visualized by TEM. SPR (surface plasmon resonance) maximum was located around 540–555 nm. An increase in the maximum SPR peak was observed as the concentration of extracts in the reaction mixtures increased. When using the extract from transgenic roots, a more heterogeneous population of nanoparticles was observed, with a larger proportion of AuNPs in the 20–40 nm size range.

Conclusions. Transformation of B. pilosa alters root metabolism, leading to increased reducing activity and affecting the process of “green” synthesis of gold nanoparticles. Extracts from both transgenic and non-transformed roots effectively promoted the synthesis of AuNPs, and increasing their concentration in the reaction mixture improved synthesis efficiency..

Keywords: Bidens pilosa L., genetic transformation, hairy roots, reducing activity, gold nanoparticles, “green” synthesis.

© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2026