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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

cover biotech acta general

Biotechnologia Acta Т. 18, No. 2, 2025
P.  , Bibliography 7 , Engl.
UDC: 616.314.17:612.08:577.115:599.323.4


PERIODONTOPROTECTIVE EFFECT OF NIMESULIDE AND NaHS UNDER SIRS CONDITIONS
Tkachenko O.T., Pletnov V.V., Mykytenko A.O.
Poltava State Medical University, Poltava, Ukraine
Accepted 2025/04/30
Hydrogen sulfide donors can protect the gastric mucosa from nimesulide damage, but their combined effect is unknown.
Aim. The work purposed to determine the concentration of peroxynitrites (ONOO-), nitrites (NO2-), and malondialdehyde (MDA) in the periodontal soft tissues of rats under the conditions of administration of nimesulide and NaHS during systemic inflammatory response syndrome (SIRS) modeling.
Materials and methods. Thirty sexually mature male Wistar rats were divided into groups: I – control, II – intraperitoneal injection with 0.4 μg/kg of bacterial lipopolysaccharide S.typhi (SIRS group), III – 18 mg/kg/day nimesulide gavage during SIRS, IV group – 5 mg/kg/day NaHS injection during SIRS, V group – NaHS and nimesulide administration during SIRS. We studied concentrations of ONOO- and NO2- and MDA.
Results and Discussion. During SIRS, ONOO- concentration in soft periodontal tissues increased by 1.12 times, NO2- by 7.02 times, and MDA by 4.51 times compared to the control (P < 0.05). Nimesulide lowers ONOO- content by 1.04 times, NO2- by 1.22 times, and MDA by 1.5 times compared to the SIRS group (P < 0.05). NaHS lowers NO2- by 11.79 times and MDA by 1.46 times compared to the SIRS group (P < 0.05). The combination of NaHS and nimesulide increases ONOO- content by 1.4 times, lowers NO2- content by 6.7 times, and MDA by 1.36 times compared to the SIRS group (P < 0.05).
Conclusions. The combination of nimesulide and NaHS lowers the intensity of lipid peroxidation in soft periodontal tissues during SIRS modeling.
Keywords: nitrites, peroxynitrite, lipid peroxidation, lipopolysaccharide, hydrogen sulfide, nimesulide.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2025