ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

Biotechnologia Acta Т. 18, No. 2, 2025
P. , Bibliography 6 , Engl.
UDC:  577: 616.03: 546.1

EFFECT OF SELENIUM CITRATE ON THE GLUTATHIONE STATUS AND THYROID HORMONES IN RATS WITH EXPERIMENTALLY INDUCED HYPOTHYROIDISM
O.O. SUSHKO1, R.JA. ISKRA
Abstract. Aim: aim of the research was to determine the effects of selenium citrate on the characteristics of the antioxidant system and hormone levels in the blood of rats with experimentally induced hypothyroidism. Materials and Methods: the hypothyroid state was induced using Mercazolil in a dose of 5 mg/kg. Hypothyroid state was determined by the levels of triiodothyronine, thyroxine, and thyroid-stimulating hormone. Animals under the research consumed selenium citrate at doses of 100 and 200 μg/kg of body weight. Results: it was found that the levels of thyroid hormones significantly decreased during hypothyroidism and had a tendency to normalize under the influence of selenium citrate. Thyroid-stimulating hormone significantly increased during hypothyroidism, though selenium compounds had no effect on its level. Changes of the activity of the glutathione status enzymes were monitored, in particular, glutathione reductase significantly increased and glutathione peroxidase decreased in animals with hypothyroidism compared to control. Selenium citrate in amounts of 100 and 200 μg/kg of body weight significantly reduced glutathione reductase activity and increased glutathione peroxidase activity compared to animals with hypothyroidism. Conclusions: the obtained results indicate a normalization of hormone levels and glutathione link indicators under the influence of selenium citrate in the blood of rats with experimentally induced hypothyroidism. These results may form the basis for the development of methods and means for the prevention and treatment of hypothyroidism.
Keywords: selenium citrate, glutathione status, hypothyroidism.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2025