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ISSN 2410-7751 (Print)
ISSN 2410-776X (on-line)

3 2016

"Biotechnologia Acta" V. 9, No 3, 2016
Р. 52-60 , Bibliography 21, English
Universal Decimal Classification: 541.49:546.732/3:547.496.26577.152.34:577.152.32

COMPLEXES OF BISCITRATOGERMANATES AND BISCITRATOSTANATES WITH METALS ARE MODIFIERS OF ACTIVITY OF Bacillus thuringiensis var. іsraelensis PEPTIDASES AND α-Penicillium canescens, Cladosporium cladosporioides AND Aspergillus niger GALACTOIDASES

L. D. Varbanets 1, N. A. Nidialkova 1, N. V. Borzova 1, I. Yi. Sayfulina 2,
O. E. Marcinko 2, O. A. Chebanenko 2

1 Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, Kyiv
2 Mechnikov Odesa National University, Ukraine

The aim of the research was to study the effect of a number of coordination compounds of stanum and germanium (compounds 1?8) as modifiers of activity of peptidases and ?-galactosidases. The coordination compounds with the same type structure of were investigated as enzymes effectors. Two types of complexes: 1) [M(H2O)6][Ge(НCitr)2]•4H2O (M = Mg(1), Mn(2), Co(3), Ni(4), Zn(5)), containing biscitrate-stanate anion ([Ge(НCitr)2]2-), and 2) [M(H2O)6][Sn(НCitr)2]•4H2O (M = Mg(6), Co(7), Ni(8)), containing biscitratostanate anion and various hexaaquacations ([M(H2O)6]2+, М= Mg, Mn, Co, Ni, Zn) were studied. It is shown that the compound 6 which is a biscitratostanate complex containing magnesium ions as metal, can be used for stimulation on 20–25% of collagenase activity of B. thuringiensis var. israelensis IMV B-7465 peptidase 1 and peptidase 2. Compound 1 (biscitrate-stanate complex containing magnesium ions as metal) and 7 (biscitratostanate complex containing cobalt ions as metal) and compound 6 in a concentration of 0.001% are able to increase elastolytic activity of peptidase 1 on 5558%. However compound 7 has shown the greatest activating effect. It increased the elastolytic activity of peptidase 2 on 100140% (for both tested concentrations). This indicates that the compound 7 can further be used as of peptidase 2 elastolytic activity effector. In the study of effect of the considered coordination compounds on the activity of α-galactosidase of Penicillium canescens, Cladosporium cladosporioides and Aspergillus niger was found that when using a number of complexes (12 and 48), there is a slight increase on 1220% of enzyme activity of P. canescens, and the maximum effect (~20%, concentration 0.01%) was provided by complex 6.

Key words: B. thuringiensis var. israelensis, ИМВ В-7465 peptidases, α-galactosidases, biscitrate-stanate and biscitratostanate complexes.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2016