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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

 6 2014

"Biotechnologia Acta" V. 7, No 6, 2014
Р. 69-74, Bibliography 16, English
Universal Decimal classification: 579.873.7:547.979.8:577.21


B. P. Matselyukh, L. V. Polishchuk, V. V. Lukyanchuk, S. A. Golembiovska, V. Y. Lavrenchuk

Zabolotny Institute of Mirobiology and Virology of the National Academy of Sciences of Ukraine, Kyiv

The aim of research was a comparative analysis of the sequences of the carotenoid biosynthetic gene clusters of the initial inactive strain S. globisporus 1912-2 and spontaneously arising, carotenoid producing mutant 1912-4Crt, and comparison of these sequences with the known sequences of the crt genes of the other representatives of streptomycetes. Streptomyces globisporus 1912 is a producer of the antitumor antibiotic landomycin E and new regulator of diketopiperazine nature. Comparative analysis of the obtained DNA sequences using the GenBank data allowed localization of 7 carotenoid biosynthetic crt genes  of  S. globisporus 1912  in  one  cluster.  This  cluster,  similar  to  other  crt clusters  of  different Streptomyces species,  consists  of  two  convergent  operons  from  4  and  3  crt genes.  The  high  homology (93%) of the crt gene clusters of S. globisporus 1912 and S. griseus IFO 13350 was shown. Two non-punctual repeats of 21 b. p. were found in the sequence of crtY gene coding lycopene cyclase. It was shown that the deletion of 117 b.p. including the sequence between non-punctual repeats of 96 b. p. and one NPR from  5’-side  activated  the  crt gene  cluster  and  production  of  beta-carotene  (6.91  mg/l)  and  lycopene (3.24 mg/l) by the mutant 1912-4Crt. A hypothesis about the site-specific recombination between two non-punctual repeats as the cause of the deletion in crtY gene of strain 1912-4Crt was proposed. The obtained strain 1912-4Crt is essential for the next genetic selection of the more effective carotenoid producers.  Deletion  of  86  b.  p.  was  revealed  in  the  regulatory  gene  lndRR  resulting  in  the  deficiency  of landomycin E production by the strain 1912-4Crt. The DNA sequences of crt and lnd genes of S. globisporus 1912 were submitted to the NCBI database with accession numbers KM349312 and KJ645792, respectively.

Key words: Streptomyces globisporus, spontaneous deletion, lycopene cyclase, crt gene cluster.

© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2014