ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
"Biotechnologia Acta" v. 7, no 4, 2014
https://doi.org/10.15407/biotech7.04.080
Р. 80-84, Bibliography 12, Russian.
Universal Decimal classification: 575.22: 58.084
Zimina O. V. 1, Sytnyk K. S. 2, Parii M. F. 2, Alkhimova O. G. 1
1Institute of Molecular Biology and Genetics of the National Academy of Sciences of Ukraine, Kyiv
2National University of Life and Environmental Sciences of the National Academy of Agrarian Sciences of Ukraine, Kyiv
The goal of the work was, using the database «The Arabidopsis Information Resource» TAIR, to select 12 SSLP-markers distributed along the Arabidopsis chromosomes and chromosome arms, to optimize the conditions of amplification of each fragment and for simultaneous amplification of several fragments. For identification of A. thaliana ecotypes and their hybrid, the SSLP sequences were used. These DNA markers are highly polymorphic in Arabidopsis and easy to use. Using this database, the primers were selected for 12 SSLP-markers distributed along all chromosomes and their arms. A. thaliana ecotypes Columbia and Landsberg erecta were used. The experiments revealed that two-stage PCR using two annealing temperatures of primers in each cycle allows efficient amplification of all the fragments considered. The conditions for carrying out two multiplex PCR, each of which allows the two fragments were amplified and a single multiplex PCR allowing three markers for amplification were defined. The developed system of DNA markers can be used to study the behavior and inheritance of each chromosome of maternal and paternal genomes of Arabidopsis hybrids and enables quick and efficient genetic analysis.
Key words: Arabidopsis thaliana ecotypes, SSLP markers, multiplex PCR.
© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2014
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