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ISSN 2410-7751 (Print)doi: 10.15407/biotech7.03.081
ISSN 2410-776X (Online)

 3 2014

"Biotechnologia Acta" V. 7, No 3, 2014
Р. 81-87, Bibliography 30, Ukrainian.
Universal Decimal classification: 577.151.03:616.379-008.64


T. I. Halenova, M. Y. Kuznetsova, O. M. Savchuk, L. I. Ostapchenko

Taras Shevchenko National University of Kyiv, Ukraine

The insulin receptor was isolated from the liver cell membranes of control and diabetic rats. The protein purity was controlled by electrophoresis and Western blot. The tyrosine kinase activity of the insulin receptor was investigated in the incubation medium in the presence of insulin (concentration range: from 10-11 M to 10-5 M). The basal tyrosine kinase activity of the insulin receptor (in the absence of insulin) was equal for the control and diabetic state. Maximal tyrosine kinase activity of the diabetic and control insulin receptor was observed at the insulin concentration of 10-8 M. Results indicate that type 2 diabetes did not cause the irregularities in the functioning of the insulin receptor that could be the reason of insulin resistance.

Key words: insulin receptor, insulin resistance, type 2 diabetes mellitus.

© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2014


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