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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

2 2014

 "Biotechnologia Acta" v. 7, no 2, 2014
Р. 34-45, Bibliography 46, English.
Universal Decimal classification: 577.112: 543.544.17(045)


О. V. Sviatenko1, O. B. Gorbatiuk2, О. А. Vasylchenko1

1National Aviation University, Kyiv, Ukraine
2State Institute of Genetic and Regenerative Medicine of National Academy of Medical Sciences  of Ukraine, Kyiv

Proteins A, G and L are native or recombinant proteins of microbial origin that bind to mammalian immunoglobulins. Preferably recombinant variants of proteins A, G, L are used in biotechnology for affinity sorbents production. Сomparative characteristics of proteins A, G, L and affinity sorbents on the basis of them, advantages and disadvantages of these proteins application as ligands in the affinity chromatography are done. Analysis of proteins A, G, L properties is presented. Binding specificities and affinities of these proteins differ between species and antibody subclass. Protein А has high affinity to human IgG1, IgG2, IgG4, mouse IgG2a, IgG2b, IgG3, goat and sheep IgG2, dog, cat, guinea pig, rabbit IgG. Protein G binds strongly to human, mouse, cow, goat, sheep and rabbit IgG. Protein L has ability of strong binding to immunoglobulin kappa-chains of human, mouse, rat and pig. Expediency of application of affinity chromatography with usage of sorbents on the basis of immobilized proteins A, G, L are shown for isolation and purification of antibodies different classes. Previously mentioned method is used as an alternative to conventional methods of protein purification, such as ion-exchange, hydrophobic interactions, metal affinity chromatography, ethanol precipitation due to simplicity in usage, possibility of one-step purification process, obtaining of proteins high level purity, multiuse at maintenance of proper storage and usage conditions. Affinity sorbents on the basis of immobilized proteins A, G, L are used not only for antibodies purification, but also for extraction of different antibodies fractions from blood serum.

Key words: affinity chromatography, Staphylococcus protein А, peptostreptococcal protein L, protein G.

© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2014


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