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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

 1 2014

"Biotechnologia Acta" v. 7, no 1, 2014
Р. 66-74, Bibliography 19, Ukrainian
Universal Decimal classification: 577.15.08: 543.9


Kucherenko I. S.1, 2, Soldatkin O. O.1, 2, Didukh D. Yu.1, 2, Soldatkin O. P.1, 2

1Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Kyiv
2Taras Shevchenko National University of Kyiv, Ukraine

Analytical characteristics of a biosensor based on glucose oxidase and hexokinase and intended for ATP determination were studied. Platinum disc electrodes were used as amperometric transducers. Range of working potentials for biosensor functioning was shown. An optimal time of enzymes immobilization was determined. Optimal conditions for biosensor functioning during work with biological fluids were selected. Biosensor work in three buffer solutions (PBS, tris and HEPES) was investigated and it was shown that it was possible to obtain various operational characteristics of the biosensor depending on tasks that are assigned to it by varying the composition of sample. Reproducibility of biosensor responses to ATP and glucose during a day and of biosensor preparation was shown. The proposed biosensor can be further used for analysis of glucose and ATP content in water solutions.

Key words: amperometric biosensor, immobilized enzymes, glucose oxidase, hexokinase, adenosine triphosphate.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2014


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