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ISSN 1995-5537

«Біотехнологія» journal V. 5, No. 5, 2012
Р. 72-81, Bibliography 45, Russian.
Universal Decimal classification: 611.451.


O. S. Sidorenkо, G. A. Bozhok, S. B. Bilyavskaya, V. D. Ustichenko, N. F. Gubina,
V. S. Kholodny, Y. I. Legach, T. P. Bondarenko

Institute for Problems of Cryobiology and Cryomedicine
of National Academy of Sciences of Ukraine, Kharkiv

The influence of culture conditions (type of substrate and composition of culture medium) on proliferation and hormone secretion in the newborn piglets adrenal primary cell culture was studied. The high level of proliferative activity accompanied by a monolayer and multicellular structures (spheroids) formation was observed when cultured in media supplemented with 10% fetal bovine serum. During further cultivation or subcultivation neuron-like cells migrated from spheroids. The process occurred in parallel with decreased cortisol secretion and did not depend on neuronal growth factor content in culture medium.

Key words: primary cell culture, adrenal gland, newborn piglets, growth factors, spheroids, cortisol, differentiation.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008