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ISSN 1995-5537

"Biotechnology" journal Vol. 4, No. 1, 2011
Р. 74-81, Bibliography 21, Ukrainian.
Universal Decimal Classification: 577.161.2:612.015.6 + 612.751


S. V. Komisarenko, L. I. Apukhovska, V. M. Riasniy, A. V. Kalashnikov, N. N. Veliky

Palladian Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv

The alimentary osteoporosis was shown to be associated with substantial (approximately 3-fold) lowering of 25-hydroxycholecalciferol (25ОНD3) levels in blood serum, suggesting reduced vitamin D3 availability. Vitamin D-hypovitaminosis causes calcium deficiency due to lack of its biologically active ultrafiltrated fraction. At the same time, the activity of alkaline phosphatase and its isoenzymes is increased in blood serum. Significant thinning of compact tissue layer and alterations in formation of osteons, inserted plates and internal surrounding bone plates were observed in femoral bones, indicating deleterious changes in the bone tissues structure. It was demonstrated nodes formation in the epiphyseal cartilage of femoral bones that occurs mainly due to the involvement of the hypertrophied cartilaginous cells. Deceleration of blood vessels growing was also seen. On the whole, these observations say for delay in osteogenesis. «Mebivid» biopharmaceutical preparation (complex of vitamin D3, calcium carbonate and bisphosphonate — methylenbisphosphonic acid disodium salt) administration to rats suffering from alimentary osteoporosis normalizes the structure and functional state of bone tissue, obviates disorders of vitamin D3 and mineral metabolism. Findings suggest the strong specific efficiency of preparation in prevention and treatment of osteoporosis.

Key words: alimentary osteoporosis, vitamin D3, bisphosphonates, methylenbisphosphonic acid disodium salt, calcium, «Mebivid» biopharmaceutical preparation.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008