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ISSN 1995-5537

3  2008

“Biotechnology” journal Vol. 1, No. 3, 2008
P. 64-71, Bibliography. 21, укр.
Universal Decimal classification:  577.15 + 543.6 + 543.9 + 543.55

Institute of Molecular Biology and Genetics, of National Academy of Sciences of Ukraine, Kyiv
Taras Shevchenko Natoinal University, Kyiv
Institute of Cell Biology, of National Academy of Sciences of Ukraine, L’viv
Ivan Franko State Pedagogical University, Drogobych

The paper presents comparative effectiveness analysis of glycerol oxidase preparations, used for development of amperometric biosensor for glycerol determination, which are different in method of preparation and in characteristics.
Enzyme preparation, whose immobilization on transducer surface results in sensor demonstrating its best operational characteristics, was selected. PH-optimum for the operation of the developed amperometric biosensor was determined; the values of buffer volume and background electrolyte concentration in buffer were shown to have no effect on the work of glycerol biosensor. The analysis of glycerol concentration in sample solutions was carried out using the developed amperometric biosensor based on glycerol oxidase.

Key words: amperometric biosensor, glycerol oxidase, glycerol, immobilization, electrochemical polymerization.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008