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ISSN 1995-5537

3  2008

“Biotechnology” journal Vol. 1, No. 3, 2008
Р. 47-55, Bibliography 26, Ukrainian.
Universal Decimal classification 579.841:577.15

Zabolotny Institute of Microbiology and Virology of the National Academy of Science of Ukraine, Kiev

The study is devoted to investigating of the basic stages of С2-compounds assimilation in Аcinetobacter sp. В-7005, to reveal «bottleneck» of С2-metabolism and to develop of approaches to their removal to improve process of microbial exopolysaccharide ethapolan synthesis. Limitation of ethanol metabolism in Acinetobacter sp. В-7005 by the rate of acetate assimilation in a reaction catalyzed by acetyl-CoA-synthetase, was shown. Basing on the investigations of С2-metabolism regulation the cultivation conditions were developed which allowed increasing the acetyl-CoA-synthetase activity in Acinetobacter sp. В-7005. Ethapolan synthesized under these conditions was characterized by high level of acylation (3?15%). Molecular mass of this polysaccharide was 1,5 million and was not changed during purification. Improved reological properties of ethapolan were associated with high molecular mass and increasing fatty acids content.
Key words: exopolysaccharides, biosynthesis, metabolism of ethanol, regulation of C2-metabolism, physical and chemical properties.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008