ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
Biotechnologia Acta Т. 17, No. 1 , 2024
P. 20-28, Bibliography 16, Engl.
UDC:: 608:576
DOI: https;//doi.org/10.15407/biotech17.01.020
Full text: (PDF, in English)
DIRECTIONS OF DEVELOPING NEW TECHNOLOGIES BASED ON CULTIVATION OF ANIMAL CELLS AND TISSUES
T.M. Lutsenko, A. S. Chernetsky, P.S. Kovalchuk, A.V. Prozor, O.O. Krailo, B.A. Ruzhytska
National Technical University of Ukraine
“Igor Sikorsky Kyiv Polytechnic Institute”
Animal cell cultures have found applications in various fields, from basic to advanced research. This includes studying the fundamentals of cell biology, mechanisms of the cell cycle, specialized cell functions, cell-cell and cell-matrix interactions, toxicity testing for the study of new drugs, gene therapy for replacing non-functional genes with functional cells, characterization of cancer cells, understanding the role of various chemical substances, viruses, and radiation in cancer cells, vaccine production, monoclonal antibodies, and pharmaceuticals. Additionally, the cultivation of viruses for use in vaccine production, such as for diseases like rabies, hepatitis B, and measles, is another important application of animal cell cultures.
Aim. The purpose of this study was to analyze the literature data on the use of animal cell lines in genetic engineering, therapy, xenotransplantation, biopharmaceuticals, the food industry, and research.
Methods. An analytical review of literature data was conducted using the information analysis of Medline (PubMed), Web of Science and Scopus databases, Google Scholar, the Cochrane Central Register of Controlled Trials (CENTRAL), and other sources up to the inclusive year 2023 using the keywords: “animal cell lines”, “immunobiological preparations”, “xenotransplantation”, “biopharmaceuticals”, “genetic engineering”.
Results. An analysis of research related to the use of animal cells in the biopharmaceutical industry was carried out, and considerations regarding the prospects for their use in various research and production technologies were outlined.
Conclusion. The technology of cultivating animal cells has become a fundamental tool in the development of research in the field of biotechnological sciences. The ability to culture animal cells in vitro has allowed the development of innovative methods, such as iPSC and organ-on-a-chip models, which have provided valuable information about disease mechanisms and potential therapeutic targets. Although there are some challenges with the use of animal cells related to variability in differentiation efficiency and concerns about safety and efficacy, further studies are needed to optimize protocols and overcome these limitations. Overall, animal cell culture technology remains an important component of modern biomedical research and has the potential to revolutionize the field of regenerative medicine.
Key words: animal cell lines, immunobiological preparations, xenotransplantation, biopharmaceuticals, genetic engineering.
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© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2024