ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
Biotechnologia Acta Т. 16, No. 6 , 2023
P. 69-75, Bibliography 23, Engl.
UDC:: 571.27; 57.083.3; 615.281.8
DOI: https://doi.org/10.15407/biotech16.06.069
Full text: (PDF, in English)
1 Taras Shevchenko National University of Kyiv, Ukraine
2 Bogomolets National Medical University, Kyiv, Ukraine
3 Latvian Biomedical Research and Study Centre, Riga, Latvia
Aim. This study aimed to examine the effect of Larifan on metabolic characteristics of human blood monocytes and granulocytes in vitro.
Methods. Four healthy adult men aged 21-26 years were recruited to participate in the study as blood donors. The metabolic profile of human blood monocytes and granulocytes was evaluated by phagocytic activity, reactive oxygen species production, nitric oxide generation, and arginase activity. Phagocytosis of FITC-labeled inactivated Staphylococcus aureus and reactive oxygen species generation were estimated by flow cytometry. Arginase activity was assessed in cell lysates, and nitric oxide generation in supernatants was examined using the Griess reaction.
Results. Phagocytic index and reactive oxygen species generation were found to be lower in both human blood monocytes and granulocytes treated with Larifan. The drug caused a dose-dependent increase in nitric oxide production, as well as a decrease in the arginase activity of blood monocytes.
Conclusions. Our results indicate the ability of Larifan to reinforce the antiviral properties of resting phagocytes along with containment of oxidative stress development.
Key words: monocytes, granulocytes, phagocytosis, reactive oxygen species, nitric oxide, arginase, metabolic polarization.
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© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2023