ISSN 2410-7751 (Print)
ISSN 2410-776X (on-line)
"Biotechnologia Acta" V. 9, No 4, 2016
https://doi.org/10.15407/biotech9.04.067
Р. 67-76, Bibliography 12, English
Universal Decimal Classification: 57.08:636:31
COMPUTER MODELING OF EMBRYONIC MORTALITY AT CRIOCONSERVATION
L. V. Gorbunov, Ye. G. Cherniavska
National Technical University “Kharkіv Polytechnic Institute”, Ukraine
The purpose of the research was to determine the regularities of influence of mammalian embryos heterogeneity and effectiveness of cryoconservation steps on their viability by using the developed simulation model. The model is based on analytical expressions that reflect the main causes of embryonic mortality during in vitro and in vivo cultivation, crioconservation and embryo transplantation. Reduction of viability depends on a set of biological factors such as the animal special, donor and recipient state, quality of embryos, and of technological ones such as the efficiency of cryopreservation method, and embryo transplantation. Fulfilled computer experiment showed, that divergence of embryos viability depending on biological parameters variations changes in a range from 0 to 100%, whereas efficiency index of chosen technology has an inaccuracy about 1%. The comparative analysis of alternative technologies of embryos cryopreservation showed the maximum efficiency of stages of use of the cryoprotectant, freezing regime and in vitro and in vivo cultivation of biological object. The application of computer modeling gives an opportunity to reduce the range of embryos viability results, obtained in different experiments is many times, thereby to shorten the time, monetary costs and the slaughter of laboratory animals in obtaining reliable results.
Key words: cryopreservation, embryos, mortality.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2016
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