ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
"Biotechnologia Acta" v. 7, no 5, 2014
https://doi.org/10.15407/biotech7.05.062
Р. 62-70, Bibliography 23, English.
Universal Decimal classification: 602.1:519.673+602.3:579.864
OPTIMIZATION OF TANNASE POSITIVE PROBIOTIC PRODUCTION BY SURFACE RESPONSE METHODOLOGY
Yumnam S.1, Prasanna B.1, Oriabinska L. B.2, Khrokalo L. A.2, Dugan O. M.2
1National Institute of Technology Karnataka, India
2National Technical University of Ukraine «Kyiv Polytechnic Institute», Kiyv, Ukraine
Study in conditions in vitro of eight Lactobacillus strains procured from culture repositories for their probiotic potential and extracellular tannase activity was the aim of the research. Based upon acid, bile salt tolerance and antibiotic resistance L. plantarum MTCC 2621 with high tannase activity was selected for production studies. Optimization of nutrient medium in 3 L bioreactor was optimized by Surface Response Methodology based on the Full Factorial Central Composite Design. A factorial design 23 augmented by 6 axial points (α= 1.68) and six replicates at the center point was implemented in 20 experiments. The optimized conditions were found to be pH 5.69, contain of lactose 128.58 g/l, peptone 8 g/l. A tenfold increase in the biomass production was observed using the optimized nutrient medium in bioreactor as compared to initial MRS medium.
Key words: Lactobacillus, optimization of medium, probiotic, response surface methodology.
© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2014
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