"Biotechnology" journal V. 5, No. 3, 2012
Р. 40-52, Bibliography 27, Ukrainian.
Universal Decimal classification: 575.23+615.222:581.143.6
Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Kyiv
Dynamic of cell systems in vitro Rauwolfia serpentina Benth tissues culture (producer of the antiarrhythmic alkaloid — ajmaline) in vitro has been described by the phenomenology equation application. Dependence of the productivity indexes dynamic as well as dynamics of cell part with different DNA content in the nucleous and different nucleoli square at passage level, and dynamics of cell part with different DNA content in the nucleous and different nucleoli square as well as dynamics of proliferation indexes (mitosis and amitosis) at circadian hierarchical level. Model construction with application of the differential characteristic has indicated that dynamic of the Rauwolfia serpentina Benth tissues culture productivity varied depending on the dynamic of cell population number with different DNA content in nucleous. Dynamic of the specific speed of alkaloid accumulation depended on dynamic cell quantity with hight DNA content in the nucleous under changed conditions (especially under stress conditions) and depended on dynamic cell quantity with 2C — 4C DNA content in the nucleous under standard conditions. According to our data the applied biotechnological methods of productivity increasing in researched cell culture have been assumed to use dynamics hereditary memory. Networks of the appropriate oscillators interaction have been constructed for different culturing condition of dynamic system of the strain K-27 cell which were phenomenology realization of the epigene network that hypothetically effected cell system self-control.
Key words: plant tissue culture, Rauwolfia serpentina, cell population in vitro, epigene network and its phenomenology realization.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008