ISSN 1995-5537
"Biotechnology" journal V. 4, No. 4, 2011
Р. 59-63, Bibliography 24, Russian.
Universal Decimal classification: 57.083.1:615.322
OPTIMIZATION OF BACTERIAL NUTRIENT MEDIA
BY Ungernia victoris EXTRACT
T. P. Pererva, A. Yu. Miryuta, A. S. Dvornik, L. P. Mozhylevskaya, V. A. Kunakh
Institute of Molecular Biology and Genetics of the National Academy of Sciences of Ukraine, Kyiv
Optimization of culture media is considered to be one of the ways to increase biomass output of bacterial cells as well as a base product at cultivation of all types and purposes.
In connection with the development of the biotechnology industrya, search of the new components of nutrient solution and their optimum ratio with the base composition of the medium and corresponding properties of the cultivated object is becoming increasingly important.
Addition of Ungernia victoris extract to bacterial nutrient medium optimizes its composition and enables to increase production of E. Coli biomass. Statistical reliable exceeding of biomass production of M17 and HB101 strains in LB medium occurs only at higher concentrations of extract (5–10%) while biomass production of JM109 strain increases at all tested concentrations (0,5–10%). The highest biomass yield of all three strains corresponds to extract concentration of 10%. The influence of U. victoris extract on growth of bacterial strains in enriched medium differs from its influence on the same strains in LB medium. Biomass increase happens at low concentrations of extract (0.25–1%)and it is not such significant as its increase in LB medium.Starting with extract concentration from 2% and to 10%, the tendency towards biomass decrease for M17 and HB101 strains and lower biomass increase for JM109 strain as compared with 0.25–1% concentrations takes place. Thus, it is possible to get improvement of bacterial biomass production by means of plant extract using both poor and enriched medium. Optimal quantity of the added extract fluctuates depending on medium composition and peculiarities of object of growth.
Key words: Escherichia coli, plant extracts, nutrient media.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008