ISSN 1995-5537
"Biotechnology" journal V. 4, No. 4, 2011
Р. 20-33, Bibliography 29, Ukrainian.
Universal Decimal classification: 577.352.4:577.164.1
Palladian Institute of Biochemistry of the National Academy of Sciences of Ukraine, Kyiv
Passive transporting of inorganic ions and organic compounds, that take part in metabolism or are the important cell constituents plays a special role in pathologies resulting from cytotoxic proteins channel creation into a target cells plasma membrane. Therefore, research of cytotoxic proteins channel creation and functioning is important for determination of a channel oligomer structure in a target cells plasma membrane and the mechanisms of cell destruction.
Cytolytic effect of protein toxins reviewed is carried out via direct transporting increase of inorganic ions through the native membranes of the target cells and release of their interior components (toxic protein channels). It is associated with formation of ion-conducting oligomers — channels in lipid bilayer of plasma membrane — by toxins. These poreforming proteins reconstruction into bilayer lipid membranes and liposomes allows to research their channel formation and functioning that provides a possibility to investigate the cytolytic protein pore structure and action mode aiming cells lysis prevention via blocking of ionic current across toxin channels. Most of the review is focused on regularity of creation and properties for relatively recent reconstructed ion-conductive oligomers of neuro- and dermatoxic β-toxin from bacteria Clostridium perfringens, another haemolytic θ-toxin of Clostridium perfringens and actoporine RTX of Radianthus macrodactilus.
Key words: bilayer lipid membrane, ion channel, Clostridium perfringens β- and θ-toxin, Radianthus macrodactilus RTX toxin.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008