FUNGAL AMYLOLITIC ENZYMES AND THEIR EFFECT UPON INSOLUBLE SUBSTRATES N. V. Borzova, E. V. Gudzenko, L. D. Varbanets

Details: Category: 6_2010(en); Hits: 181

ISSN 1995-5537

"Biotechnology" journal Vol. 3, No. 6, 2010
Р. 36-41, Bibliography 9, Ukrainian.
Universal Decimal classification: 577.152.34

FUNGAL AMYLOLITIC ENZYMES
AND THEIR EFFECT UPON INSOLUBLE SUBSTRATES

N. V. Borzova, E. V. Gudzenko, L. D. Varbanets

Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, Kyiv

Effective strains displaying glucoamylase activity have been obtained as a result of screening among the representatives of genera Acremonium, Alternaria, Eupenicillium, Gliocladium, Myrothecium, Penicillium, Rhizopus. However, the most active biosynthetics belong to Aspergillus, Fusarium and Phoma. The studies of a number of cultivation conditions of four Aspergillus sp. strains indicated that the optimum sources of carbon at its submerged cultivation were 2% potato or corn starch, and also the mixture of 2% potato starch with 1% maltose. The comparative effects of glucoamylase and amylase preparations in saccharofication of insoluble substrates containing starch have been studied.

Key words: glucoamylase, amylase, screening, carbon sources, hydrolysis of insoluble substrates.