ISSN 1995-5537
"Biotechnology" journal Vol. 3, No. 6, 2010
Р. 85-91, Bibliography 13, Ukrainian.
Universal Decimal classification: 577.1
ESTIMATION OF CYTOTOXICITY DEGREE OF PATHOLOGICAL SERUM COMPONENTS USING CELLULAR TEST-SYSTEM
E. M. Klimova, E. V. Lavinskaya, A. I. Bozhkov, T. I. Kordon
National Enterprise «Institute of general and urgent surgery
of Academy of Medical Sciences of Ukraine», Kharkiv;
V. N. Karazin’s Research Institute of Biology, Kharkiv National University, Kharkiv
Screening indication of blood serum cytotoxicity with a test-system based on monocellular algae Dunaliella viridis was carried out. Significant differences of morphological and functional parameters D. viridis, induced by cytotoxic factors of various nature were detected. Patterns of morphological and functional changes of the bioindicator cells in the coordinate system were defined. Coefficients of toxicity for investigated biological liquids are calculated. Maximum degree of cytotoxicity was revealed in blood serum received from the patients suffering from biliary cirrhosis (КЦ = 13.01±01) and myasthenia (КЦ = 11.6±0.76), minimal one (КЦ = 5.65±0.66) was with acute varicothrombosis.
Key words: cellular test-system, cytotoxicity, bioindication, screening, pattern.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008