1_2017(en)

Details: Hits: 117

"Biotechnologia Acta" V. 10, No 1, 2017
https://doi.org/10.15407/biotech10.01.061
Р. 61-67, Bibliography 17, English
Universal Decimal Classification: 577.112:616.128

1 2017

PT (II) AND PD (II) COMPLEXES INFLUENCE ON SPHEROIDS GROWTH OF BREAST CANCER CELLS

A. A. Bilyuk ¹, O. V. Storozhuk ¹, O. V. Kolotiy ¹, H. H. Repich ², S. I. Orysyk ², L. V. Garmanchuk ¹

¹ Educational and Scientific Centre “Institute of Biology and Medicine” of Taras Shevchenko National University of Kyiv, Ukraine
² Vernadskyi Institute of General and Inorganic Chemistry of the National Academy of Sciences of Ukraine, Kyiv

The aim of the research was to examine the changes in multi-cellular tumor spheroid growth, adhesion properties and gamma-glutamintranspeptidasic activity in model systems of human breast cancer multicellular spheroid MCF-7 under the influence of Pt(ІІ) and Pd(ІІ) π-complexes with allyl-containing thioureas. Comparing with cisplatin, Pt(II) and Pd(II) complexes reduce gamma-glutamintranspeptidasic activity, increase adhesive properties in model system of solid tumor and inhibit the multicellular spheroids’ growth. All changes prove the importance of further investigation and analysis of these compounds as potential analogues of anticancer drugs that possibly do not cause resistance and reduce the level of metastasis in breast cancer.

Key words: Pt(ІІ) and Pd(ІІ) π-complexes, gamma-glutamintranspeptidase, adhesive properties, multi-cellular tumor spheroids.

References

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Details: Hits: 171

"Biotechnologia Acta" V. 10, No 1, 2017
https://doi.org/10.15407/biotech10.01.052
Р. 52-60, Bibliography 41, English
Universal Decimal Classification: 57.065.38

1 2017

SYNTHESIS AND CHARACTERIZATION OF POLY(LACTIC-CO-GLYCOLIC-ACID) MICROPARTICLES
LOADED WITH FOOT-AND-MOUTH DISEASE VIRUS
40–60 SYNTHETIC PEPTIDE

Z. Mustafaeva

Yildiz Technical University, Chemical and Metallurgy Faculty, Istanbul, Turkey

The aim of the research was to synthesize and characterize the poly (lactic-co-glycolic-acid) microparticles loaded with foot-and-mouth disease virus 40-60 synthetic peptide. Single emulsion solvent evaporation method was used for the production of microparticles with theree diferent (5; 10 and 15 mg) peptide amounts and obtained microparticles were characterized by using yield reaction, encapsulation efficiency, drug loading, particle size, polydispersity index, and zeta potential.

Key words: virus of the foot-and-mouth disease, poly (lactic-co-glycolic-acid) microparticles.

References

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27. Arasoglu T., Mansuroglu B., Derman S., Gumus B., Kocyigit B., Acar T., Kocacaliskan I. Enhancement of Antifungal Activity of Juglone (5-Hydroxy-1, 4-naphthoquinone) Using a Poly (d, l-lactic-co-glycolic acid)(PLGA) Nanoparticle System. J. Agricult. Food Chem. 2016, 64 (38), 7087–7094. https://doi.org/10.1021/acs.jafc.6b03309

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31. Chong C. S., Cao M., Wong W. W., Fischer K. P., Addison W. R., Kwon G. S., Tyrrell D. L., Samuel J. Enhancement of T helper type 1 immune responses against hepatitis B virus core antigen by PLGA nanoparticle vaccine delivery. J. Controll. Release. 2005, 102 (1), 85–99. https://doi.org/10.1016/j.jconrel.2004.09.014

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34. Ma W., Chen M., Kaushal S., McElroy M., Zhang Y., Ozkan C., Bouvet M., Kruse C., Grotjahn D., Ichim T. PLGA nanoparticle-mediated delivery of tumor antigenic peptides elicits effective immune responses. Intern. J. Nanomed. 2012, V. 7, P. 1475. https://doi.org/10.2147/IJN.S29506

35. Primard C., Poecheim J., Heuking S., Sublet E., Esmaeili F., Borchard G. Multifunctional PLGA-based nanoparticles encapsulating simultaneously hydrophilic antigen and hydrophobic immunomodulator for mucosal immunization. Mol. Pharmaceut. 2013, 10 (8), 2996–3004. https://doi.org/10.1021/mp400092y

36. Silva A., Rosalia R., Sazak A., Carstens M., Ossendorp F., Oostendorp J., Jiskoot W. Optimization of encapsulation of a synthetic long peptide in PLGA nanoparticles: Low-burst release is crucial for efficient CD8(+) T cell activation. Europ J. Pharmaceut. Biopharmaceut. 2013, 83 (3), 338–345. https://doi.org/10.1016/j.ejpb.2012.11.006

37. Taha M. A., Singh S. R., Dennis V. A. Biodegradable PLGA85/15 nanoparticles as a delivery vehicle for Chlamydia trachomatis recombinant MOMP-187 peptide. Nanotechnology. 2012, 23 (32), 325101. https://doi.org/10.1088/0957-4484/23/32/325101

38. Xiao X., Zeng X., Zhang X., Ma L., Liu X., Yu H., Mei L., Liu Z. Effects of Caryota mitis profilin-loaded PLGA nanoparticles in a murine model of allergic asthma. Intern. J. Nanomed. 2013, V. 8, P. 4553.

39. Pawar D., Mangal S., Goswami R., Jaganathan K. Development and characterization of surface modified PLGA nanoparticles for nasal vaccine delivery: effect of mucoadhesive coating on antigen uptake and immune adjuvant activity. Europ. J. Pharmaceut Biopharmaceut. 2013, 85 (3), 550–559. https://doi.org/10.1016/j.ejpb.2013.06.017

40. Manish M., Rahi A., Kaur M., Bhatnagar R., Singh S. A single-dose PLGA encapsulated protective antigen domain 4 nanoformulation protects mice against Bacillus anthracis spore challenge. PLOS ONE. 2013, 8 (4), e61885. https://doi.org/10.1371/journal.pone.0061885

41. Jaganathan K., Vyas S. P. Strong systemic and mucosal immune responses to surface-modified PLGA microspheres containing recombinant hepatitis B antigen administered intranasally. Vaccine. 2006, 24 (19), 4201–4211. https://doi.org/10.1016/j.vaccine.2006.01.011

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"Biotechnologia Acta" V. 10, No 1, 2017
https://doi.org/10.15407/biotech10.01.043
Р. 41-51, Bibliography 16, English
Universal Decimal Classification: 612.397:661.725.4

1 2017

THE CONTENTS OF NEUTRAL AND POLAR LIPIDS IN CLOSTRIDIA CELLS UNDER CULTIVATION IN THE PRESENCE OF BUTANOL

S. I. Voychuk

Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, Kyiv

The aim of the study was to evaluate changes in the portion of polar and neutral lipids in the cells of Clostridium during their cultivation in the presence of butanol. Four natural isolates of Clostridium genus were studied with flow cytometry approaches. Under the optimal culture conditions, the polar lipids prevailed over neutral ones in bacterial cells; the content of neutral lipids doubled in spores of these microorganisms, while the content of polar ones was reduced. Strains No 1 and No 2 were able to grow at 1% butanol in the medium, and the strain No 4 was at 1.5%. When cultivated in the presence of different concentrations of butanol, the bacterial strains did not differ in such cytomorphological features as granularity and cell size. The quantitative content of polar and neutral lipids in the presence of butanol varied depending on the content of butanol in the medium, however this effect had a strain-specific character and did not show a correlation with the resistance of these bacteria to butanol. So, the content of polar and neutral lipids varied depending on butanol content in the medium. However this effect was strain-specific independently of resistance of these bacteria to butanol. The use of bacterial biomass as a source of lipids for the production of biofuels requires further optimization of the process to increase the content of the neutral lipid fraction in bacterial cells.

Key words: Clostridium, polar and neutral lipids, butanol, flow cytometry.

References

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2. Guan Z., Tian B., Perfumo A., Goldfine H. The polar lipids of Clostridium psychrophilum, an anaerobic psychrophile. Biochimica et biophysica acta. 2013, 1831(6), 1108-1112.https://doi.org/10.1016/j.bbalip.2013.02.004

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7. De la Jara, A., Mendoza, H., Martel, A., Molina, C., Nordstron, L., de la Rosa, V., D?az, R. Flow cytometric determination of lipid content in a marine dinoflagellate, Crypthecodinium cohnii. J. Appl. Phycol. 2003, 15, 433-438. https://doi.org/10.1023/A:1026007902078

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9. Adler H.I., Crow W. A technique for predicting the solvent-producing ability of Clostridium acetobutylicum. Appl. Environ. Microbiol. 1987, 53(10), 2496–2499. PMC204135.

10. Tracy B.P., Gaida S.M., Papoutsakis E.T. Development and application off-cytometric techniques for analyzing and sorting endospore-forming clostridia. Appl. Environ. Microbiol. 2008, 74(24), 7497–7506. https://doi.org/10.1128/AEM.01626-08

11. Neumeyer A., H?bschmann T., M?ller S., Frunzke J. Monitoring of population dynamics of Corynebacterium glutamicum by multiparameter flow cytometry. Microbial Biotechnology. 2013, 6(2), 157–167. https://doi.org/10.1111/1751-7915.12018

12. Jones S.W., Tracy B.P., Gaida S.M., Papoutsakis E.T. Inactivation of ?^F in Clostridium acetobutylicum ATCC 824 blocks sporulation prior to asymmetric division and abolishes ?^E and ?^G protein expression but does not block solvent formation. J. Bacteriol. 2011, 193(10), 2429–2440. https://doi.org/10.1128/JB.00088-11

13. Tracy B.P., Jones S.W., Papoutsakis E.T. Inactivation of ?^E and ?^G in Clostridium acetobutylicum illuminates their roles in clostridial-cell-form biogenesis, granulose synthesis, solventogenesis, and spore morphogenesis. J. Bacteriol. 2011, 193(6), 1414–1426. https://doi.org/10.1128/JB.01380-10

14. Kolek J., Branska B., Drahokoupil M., Patakova P., Melzoch K., Sauer M. Evaluation of viability, metabolic activity and spore quantity in clostridial cultures during ABE fermentation. FEMS Microbiol. Lett. 2016, 363(6): fnw031. https://doi.org/10.1093/femsle/fnw031

15. Skrotska O.I., Penchuk Yu.M., Skrotskuy S.O., Gavrulenko M.M., Smirnova G.F. A selection of acetone-butanol bacteria is from different natural sources. Food Industry, 2010, 9:49-52. URI: dspace.nuft.edu.ua/jspui/handle/123456789/867

16. Kujawska A., Kujawski J., Bryjak M., Kujawski W. ABE fermentation products recovery methods - A review. Renewable and Sustainable Energy Reviews. 2015, 8(8), 648-661. https://doi.org/10.1016/j.rser.2015.04.028

Details: Hits: 139

"Biotechnologia Acta" V. 10, No 1, 2017
https://doi.org/10.15407/biotech10.01.034
Р. 34-42, Bibliography 54, English
Universal Decimal Classification: 579.222

1 2017

DOMESTIC BUTANOL-PRODUCING STRAINS OF THE Clostridium GENUS

O. O. Tigunova, N. Ye. Beiko, H. S. Andriiash, S. H. Pryiomov, S. M. Shulga

«Institute of Food biotechnology and genomics of the National Academy of Sciences of Ukraine», Kyiv

The aim of the work was to summarize the results of own research concerning obtaining butanol producing strains of Clostridium genus, to identify them by physiological, morphological and genetic methods. Further study of characteristics and biological features of the strains, and various approaches in biotechnological process of butanol production are discussed. The work includes methods to increase butanol accumulation by producer strains. Perspectives of using chemical mutagenesis in Clostridia as a method of increasing butanol production are considered. The feasibility of using non-food raw material as a substrate for fermentation is discussed. Different methods of pretreatment and their impact on the accumulation of butanol in the liquid medium are compared. Butanol accumulation is shown to increase significantly if the synthesis precursors are added as components of enzymatic medium, and the “reverse bard” is used to reduce waste production without affecting the level of butanol synthesis. The problem of conservation of producing strains is given, and protective medium for microorganisms during the freeze-drying is defined.

Key words: butanol, butanol-producing strains of the Clostridium genus.

References

1. Abd-Alla M. H., Zohri A.-N. A., El-Enany A.-W. E., Ali S. M. Acetone-butanol-ethanol production from substandard and surplus dates by Egyptian native Clostridium strains. Anaerobe. 2015, V. 32, P. 77–86. https://doi.org/10.1016/j.anaerobe.2014.12.008

2. Menon N., Paszotor A., Menon B. R. K., Kallio P., Fisher K., Akhtar M. K., Leys D., Jones P. R., Scrutton N. S. A microbial platform for renewable propane synthesis based on a fermentative butanol pathway. Biotechnol. Biofuels. 2015, 8 (61), 1–12. https://doi.org/10.1186/s13068-015-0231-1

3. Linggang S., Phang L. Y., Wasoh H., Abd-Aziz S. Acetone-butanol-ethanol production by Clostridium acetobutylicum ATCC 824 using sago pith residues hydrolysate. BioEnergy Res. 2013, 6 (1), 321–328. https://doi.org/10.1007/s12155-012-9260-9

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Details: Hits: 98

"Biotechnologia Acta" V. 10, No 1, 2017
https://doi.org/10.15407/biotech10.01.026
Р. 26-33, Bibliography 22, English
Universal Decimal Classification: 577. 152.32

1 2017

YEAST β-MANNANASE ACTIVITY

N. V. Borzova, L. D. Varbanets, V. S. Pidgorskyi, O. D. Ianieva

Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, Kiyv

The aim of the research was to determine the mannan-degrading activity of yeasts cultures isolated from various sources and select strains with high β-mannanase activity. As a result of screening of 245 yeast strains, which are the representatives of 7 genera and 14 species, the active producers of extracellular β-mannanase were identified. To increase β-mannanase activity, the cultures were grown under submerged conditions using guar gum galactomannan as a carbon source and an inducer. β-Mannanase activity was determined by dinitrosalicylic method. The most active biosynthetic species were Cryptococcus albidus, C. gastricus, C. magnus, C. terreus, C. laurentii, Saccharomyces cerevisiae, Williopsis californica, Metschnikowia pulcherrima, Pichia anomala and P. guilliermondii. The activity in culture supernatant was ranged from 0.2 to 75 U/ml. α-Galactosidase activity was found in two strains (Debaryomyces polymorphus UCM Y-152 and Debaryomyces hansenii var. fabryi UCM Y-2400). None of the tested cultures demonstrated both β-mannanase and α-galactosidase activity, that is, they are unable to attack both the main and side chains of galactomannan.

Key words: yeast, β-mannanase, α-galactosidase, galactomannan.

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