3_2009(en)

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ISSN 1995-5537

"Biotechnology" journal Vol. 2, No. 3, 2009
Р. 55-63, Bibliography 37, Ukrainian.
Universal Decimal classification 602.68

CHARACTERIZATION OF scFv-ANTIBODIES AGAINST DIPHTHERIA TOXIN, ISOLATED FROM IMMUNE AND NA?VE IMMUNOGLOBULIN GENE LIBRARIES

O. S. Oliinyk, D. V. Kolibo, S. V. Komisarenko

Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, Kyiv

The diphtheria toxin plays the leading role in the development of diphtheria infection that's why antibodies against toxin are extensively used in the diagnostics and therapeutics of diphtheria. We have previously selected a number of scFv-antibodies against diphtheria toxin B subunit from immune and naive immunoglobulin library. The aim of this work was to characterize some important properties of obtained scFv-antibodies.

It was confirmed by restriction endonuclease cleavage analysis that the scFv-antibodies selected from immune library after single round of selection were diverse and all selected sequences were unique. The scFv-antibodies selected from immune library were specific exactly to sequence of diphtheria toxin B fragment. Data obtained by Western blot analysis of clones derived after subcloning of scFv’s DNA-sequences into рЕТ-22b vector confirmed that target protein expressed mainly in insoluble fraction. These results confirmed that we selected a number of clones-producers of heterogeneous scFv-antibodies specific to sequence of diphtheria toxin. This scFv could be used for anti-diphtheria diagnostic and therapeutic drugs of the new generation.

Key words: scFv-antibodies, diphtheria toxin, naive and immune immunoglobulin, genes libraries.

Details: Hits: 85

ISSN 1995-5537

"Biotechnology" journal Vol. 2, No. 3, 2009
Р. 9-22, Bibliography 102, Russian.
Universal Decimal classification 577.157.2

RECOMBINANT ANTICOAGULANT FORM OF A HUMAN α-THROMBIN

M. V. Kolodzejskaja, V. I. Grishchuk, T. M. Chernyshenko, E. I. Jusova, T. V. Grinenko

Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine

Mutant thrombin forms that cause essential changes of an active center geometry, blocking of the subsites of the substrata linkage located in separate loops of a molecule of enzyme that results in change of conformational mobility of a molecule and hence an increased activity of enzyme are discussed in the review. Question concerning thrombin allostery is important for identification of interrelation between its structure and thrombin functions and also for development of a new direction in therapy.

Key words: recombinant thrombin, allostery, mutagenesis, protein C, thrombomodulin, anti coagulant function.

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ISSN 1995-5537

"Biotechnology" journal Vol. 2, No. 3, 2009
Р. 23-28, Bibliography 12, Ukrainian.
Universal Decimal classification 577.112.7

GREEN FLUORESCENT PROTEIN AND ITS ANALOGUES

A. P. Demchenko

Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine

Unique properties of fluorescent proteins to synthesize and fold spontaneously with formation of the fluorescent groups have attracted attention of many researchers and led to creation of revolutionary new technologies in cell studies. In this review the properties of green fluorescent protein of Aequorea Victoria have been analyzed. Presently, many proteins with these unique properties were synthesized, which allows their application as potent intracellular molecular labels and sensors.

Key words: fluorescent proteins, markers of protein synthesis, label of cell proteins, intracellular sensors.

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ISSN 1995-5537

"Biotechnology" journal Vol. 2, No. 3, 2009
Р. 29-43, Bibliography 32, Russian.
Universal Decimal classification 547.426.2.057

BIOLOGICAL ACTIVITY OF PHOSPHORUS
AND NON7PHOSPHORUS ALKYL GLYCEROLIPIDS

S. G. Romanova, V. G. Romanov, G. O. Serebrennikova

Lomonosov Moscow State Academy of Thin Chemical Technology, Moscow

The review is related to modern investigations of biological activity of phosphorus and non-phosphorus alkyl glycerolipids. Based on the experimental articles published last decade the questions on stereospecific ether glycerolipids and their biological properties such as antineoplastic activity, ability to inhibit protein kinase C, PAF- antagonistic effect, anti-HIV action and possibility of their use in biotechnology especially in gene therapy are considered in the review.

Key words: ether glycerolipids; edelfosine; biological activity of alkyl glycerolipids.

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ISSN 1995-5537

"Biotechnology" journal Vol. 2, No. 3, 2009
Р. 44-54, Bibliography 101, Ukrainian.
Universal Decimal classification 612.393:577.151.6+547.972

SOYBEAN ISOFLAVONES: BIOLOGICAL ACTIVITY AND APPLICATION

V. A. Baraboy

Bremen, Germany

Some structural pecularities of soybean isoflavones determine their biological activity: antioxidant, inhibitory, weak estrogenic and antiestrogenic (because of similarity with estrogen hormones). Isoflavones are nontoxic, soy is used as a component of commercial infant dietes. High consumption of soy in Japan and China is a reason of low cardiovascular and oncologic pathology. Antioxidant and estrogenic effects of isoflavones provide normalization of cholesterol, triglyceride level, lipoprotein peroxidation, normalization of lipid metabolism. Inhibiting action of ispoflavone on tyrosinkinase, topoisomerase, ornitindecarboxilase activity, antiproliferative effect limit the induction and development of some tumors, especially hormonodependent.

Key words: soybean isoflavones, biological activity, practical application.