2_2009(en)

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ISSN 1995-5537

"Biotechnology" journal Vol. 2, No. 2, 2009
Р. 111-117, Bibliography 16, Ukrainian.
Universal Decimal classification: 615.373: 535..394:620.266.1

T-2 TOXIN DETERMINATION BY SURFACE PLASMON RESONANCE

О. S. Gojster, G. O. Khmel’nitsky, S. V. Dzyadevych, W. I. Nasarenko, O. Н. Minchenco

Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv;
National Agricultural University, Kyiv

Institute of Molecular Biology and Genetics,National Academy of Sciences of Ukraine, Kyiv

A possibility of application of optimal immune biosensor based on surface plasmon resonance for express determination of trichotecen mycotoxin T-2 in solution has been investigated. Two variants of T-2 toxin determination by surface plasmon resonance immunosensor method have been proposed. It was shown opportunity of «direct» determination of T-2 with the detection limit of about 20 ng/ml and for «competitive» mode with the detection limit on the level of about 1 ng/ml. The effectiveness of application of such organic compounds as polyelectrolytes and thiols for sensible surface modification of SPR spectrometer for T-2 analysis in solutions was studied. Direct determination by using dodecanethiol and polyelectrolyte for surface modification allows to determine T-2 toxin at levels of 5 ng/ml and 2 ng/ml accordingly in case of using both poly- and monoclonal antibodies.

Key words: immobilization, T2 toxin, surface plasmon resonance, polyelectrolyte, dodecantiol.

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ISSN 1995-5537

"Biotechnology" journal Vol. 2, No. 2, 2009
Р. 9-22, Bibliography 95, Russian.
Universal Decimal classification: 615.37: 615.246.2

IMMUNOADSORРTION FOR THERAPY OF AUTOIMMUNE DISEASES

K. I. Bardakhivska, N. M. Gurina, T. M. Kuchmerovska, V. G. Nikolaev

R. E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology
National Academy of Sciences of Ukraine, Kyiv
O. V.Palladin Institute of Biochemistry
National Academy of Sciences of Ukraine, Kyiv

In review, application of immunoadsorption for therapy of autoimmune diseases is elucidated. It was focused on the most widespread autoimmune diseases, such as rheumatoid arthritis, systemic lupus erythematosus, multiple sclerosis and other. Current knowledge concerning pathogenesis of autoimmune diseases was also covered. Treatment of these diseases due to coplexity of the underlying mechanisms requires new approaches besides using traditional therapy. Growing evidence suggests that such approaches may include immunoadsorption. It was demonstrated that immunoadsorption may be applied as main or additive method in combined therapy of autoimmune diseases due to its high efficacy and safety.

Key words: immunoadsorbtion, immunosorbent, autoimmune diseases.

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ISSN 1995-5537

"Biotechnology" journal Vol. 2, No. 2, 2009
Р. 42-54, Bibliography 34, Ukraininan.
Universal Decimal classification: 577.152.32

MICROBIAL α-GLUCOSIDASES: CLASSIFICATION, SUBSTRATE SPECIFICITY AND MECHANISM OF ACTION

N. V. Borzova

Institute of Microbiology and Virology of National Academy of Sciences of Ukraine, Kyiv

The review focuses on systematic identification of microbial α-glucosidases in present enzyme nomenclature based on substrate specificity and molecular structure. Particular attention is given to α-glucosidase occurrence among microorganisms, including extremophiles, their physical and chemical properties, catalytical abilities, substrate specificity and mechanism of action.

Key words: α-glucosidase, classification, properties, specificity, mechanism of action.

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ISSN 1995-5537

"Biotechnology" journal Vol. 2, No. 2, 2009
Р. 55-66, Bibliography 62, Ukrainian.
Universal Decimal classification: 546.26.043

CARBON NANOTUBES AS A NEW CLASS OF MATERIALS FOR NANOBIOTECHNOLOGY

S. V. Prylutska, О. V. Remeniak, Yu. V. Honcharenko, Yu. I. Prylutskyy

Кyiv National Taras Shevchenko University

The data concerning physical and chemical properties of carbon nanotubes, their biocompatibility, cytotoxicity, membranоtropic and thermal action that provide their use in biotechnology and medicine have been generalized.

Кey words: single and multiwalled carbon nanotubes, cytotoxicity, biocompatibility, membranetropic action, nanobiotechnology.

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ISSN 1995-5537

Biotechnology" journal Vol. 2, No. 2, 2009
Р. 67-77, Bibliography 103, Russian.
Universal Decimal classification: 678.632

GRAPE PHENOLS: STRUCTURE, ANTIOXIDANT ACTIVITY APPLICATIONS

V. A. Baraboy

Bremen, Deutschland

Phenolic substances of grape, of red and white vins and juices — flavonoids (anthocyanidins, catechins, flavonols) and phenolic acids — are powerful antioxidants. Resveratrol and other oxystilben phytoalexins synthetize after attack of pathogene mushrooms. The anthocyans from red grape and vines possess the most antioxidant activity — and antisclerotic activity (the «French paradox»). The most active antioxidants cyanidin, malvidin and its glucosodes.

The white vines (juices) maintain 7–10 once less phenols than red vines but they have high antioxidant activity as well. The grape phenols shou antitoxic, hepatoprotective, antimutagenic and anticancerogenic activity.

Key words: grape, phenolic substances, antioxidant activity.