ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
"Biotechnologia Acta" v. 6, no. 3, 2013
Р. 63-68, Bibliography 12, Russian.
Universal Decimal classification: 57.085.2
PROPERTIES OF ISOLATED NEURAL CELLS FROM NEWBORN RAT IN TISSUE in vitro
A. N. Sukach1, 2, T. D. Liashenko2, M. V. Shevchenko2
1Institute for Problems of Cryobiology and Cryomedicine
of National Academy of Sciences of Ukraine, Kharkiv
2Skovoroda National Pedagogical University, Kharkiv, Ukraine
It is shown that isolated brain cells of newborn rats are heterogeneous suspension consisted of differentiated cells, stem and progenitor cells, which can proliferate and differentiate in conditions of in vitro cultivation. During cultivation in presence of serum the neural cells of the newborn rats form aggregates, where reconstitution of cellular microenvironment lost in isolation takes place followed by cell reparation. After attachment to a substrate the aggregate cells migrate and spread, form a monolayer of astroglia, which the neuroblasts and colonies of stem/progenitor cells are formed on. The findings show feasible application of newborn rat neural cells as model of postnatal neural tissue for studying the influence of cellular environment on process of development and recovery of differentiated neural cells, as well as proliferation and differentiation of stem/progenitor cells.
Key words: differentiated nervous cells, nervous stem/progenitor cells, new-born rats, viability, survival, cultivation, multicellular aggregates.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008
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