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- Category: 6_2014(en)
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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
"Biotechnologia Acta" v. 7, no 6, 2014
https://doi.org/10.15407/biotech7.06.029
Р. 29-39, Bibliography 32, English
Universal Decimal classification: 577.322, 577.152.271
EFFECT OF INHIBITORS OF PROTEIN KINASE CK2 ON THE ACTIVITY ITS CATALYTIC SUBUNITS СКα AND СК2α′
O. V. Ostrynska, O. P. Kukharenko, V. G. Bdzhola, S. M. Yarmoluk
Institute of Molecular Biology and Genetics of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
The effect of protein kinase CK2 inhibitors (with IC50 from 0.004 μM to 0.7 μM) from different chemical classes on the activity of СК2α and СК2α′ recombinant proteins has been studied. Biochemical tests shown that isozymes had different sensitivity toward the same compounds. The most isoform-selective inhibitor was 4′-hydroxyflavone derivative (FLC26) with IC50 value 0.020 μM (CK2α) and 0.003 μM (CK2α′). To explain the difference between influences of FLC26 on the activities of two CK2 catalytic subunits their complexes with ATP-binding site of CK2α and CK2α′ were analyzed using molecular modeling techniques. The data obtained by molecular dynamics simulation (10 ns) has not provided a clear explanation of the difference between the inhibitory potency of the compound FLC26 towards the CK2 catalytic subunits. Thus, the reasons underlying the different activities of the same inhibitor on CKα and CKα′ require further investigations. An effective approach for this purpose would be X-ray analysis of complexes "compound FLC26- CKα/CKα′".
Key words: catalytic subunits of protein kinase CK2, docking, molecular dynamics simulation.
© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2014
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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
"Biotechnologia Acta" v. 7, no 6, 2014
https://doi.org/10.15407/biotech7.06.023
Р. 23-28, Bibliography 11, Russian
Universal Decimal classification: 577.158.54
THE THERMAL INACTIVATION OF Eupenicillium erubescens α-L-RHAMNOSIDASE
O. V. Gudzenko, N. V. Borzova, L. D. Varbanets
Zabolotny Institute of Microbiology and Virology
of the National Academy of Sciences of Ukraine, Kiev, Ukraine
The study of the features of thermal inactivation of α-L-rhamnosidase from Eupenicillium erubescens aiming to develop further strategies for improving the stability of the enzyme was the purpose of the research. Two forms of α-L-rhamnosidases were studied. They were obtained by producer cultivation on two different carbon sources (rhamnose and naringin). It was shown that at temperatures above 60 0C heat inactivation process is described by the first order reaction kinetics. It was established that stability of α-L-rhamnosidase from E. erubescen, grown on naringin depends on hydrophobic interactions rather than electrostatic ones. It was shown that aggregation of enzyme molecules could lead to their thermal stabilization. The important role of cysteine residues and possible involvement of the metal cation in maintaining of active form of α-L-rhamnosidases from E. erubescens was highlighted.
Key words: Eupenicillium erubescens, α-Lrhamnosidase, thermal inactivation, naringin, rhamnose.
© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2014
References
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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
"Biotechnologia Acta" v. 7, no 6, 2014
https://doi.org/10.15407/biotech7.06.017
Р. 17-22, Bibliography 14, English
Universal Decimal classification: [597-146.3:597-146.5]:597.553.2
THE RAPID DIAGNOSTICS OF SEX OF SALMONIDS USING DNA-MARKERS
1Institute of Fisheries of the National Academy of Agrarian Sciences of Ukraine, Kyiv
2Taras Shevchenko National University of Kyiv, Ukraine
Based on nucleotide sequences of sex-specific DNA-markers of salmonid fishes the oligonucleotide primers for polymerase chain reaction were selected with purpose on rapid diagnostic of sex in rainbow trout Onchorhynchus mykiss, brown trout Salmo trutta, huchen Hucho hucho and grayling Thymallus thymallus. The specify of amplification was determined by nucleotide sequence analysis of PCR-products. All amplified fragments were referred to sex-specific locuses of Y chromosomes in males of investigated fish species. The PCR-products were in size of 880, 607, 521 and 558 for rainbow trout, brown trout, grayling and huchen respectively. Thus the sex determination in above mentioned fish species and identification of genotypic males under process of hormonal sex reversion can be provided using conventional PCR. Present method relates to rapid diagnostics because the data analysis and return of results back to fish farm take one single day.
Key words: DNA-markers, PCR diagnostics, sex reversion, salmon raising.
© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2014
References
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14. Matsuda M., Nagahama Y., Shinomlya A., Sato T., Matsuda C., Kobayashi T., Morrey C. E., Shibata N., Asakawa S., Shimizu N., Hori H., Hamaguchi S., Sakaizumi M. DMY is a Y-specific DM-domain gene required for male development in the medaka fish. Nature. 2002, V. 417, P. 559–563.
http://dx.doi.org/10.1038/nature751
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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
"Biotechnologia Acta" v. 7, no 6, 2014
Р. 9-16, Bibliography 32, Ukrainian
Universal Decimal classification: 571.27:004.9
https://doi.org/10.15407/biotech7.06.009
In silico DETERMINATION OF T-EPITOPES OF Mycobacterium tuberculosis PROTEINS
O. I. Krynina, D. V. Kolibo, S. V. Komisarenko
Palladian Institute of Biochemistry of the National Academy of Sciences of Ukraine, Kyiv
The comparative analysis of 8 antigens of Mycobacterium tuberculosis (MPT83, MPT63, ESAT-6, CFP10, SodA, leuD, panD and HBHA) in silico to determine T-epitopes that can bind MHC class II molecules coding the most common in Eastern Europe HLA-DRB1 alleles was the goal of the work.
The results show that the most immunogenic proteins are SodA, MPT83, leuD and MPT63, which has the largest number of T-epitopes for the alleles of interest. Accordingly, these antigens or their combinations could be promising candidates for development of new vaccines and diagnostics to form anti-TB immunity in Eastern European region and to determine the state of protection of the population.
Key words: Mycobacterium tuberculosis, T-epitope.
© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2014
References
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