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ISSN 2410-776X (Online),
ISSN 2410-7751 (Print)

 1 2015

Biotechnologia Acta, V. 8, No 1, 2015; 
https://doi.org/10.15407/biotech8.01.032
P. 32-38, Bibliography 12, English
Universal Decimal classification: 579.841.11.22

SEROLOGICAL AND BIOLOGICAL ACTIVITY OF LIPOPOLYSACCHARIDE
FROM Escherichia coli L-19

L. D. Varbanets 1, E. L. Zdorovenko 2, Е. G. Garkavaya 3,  O. S. Brovarskaya 1

1 Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
2 Zelinsky Institute of Organic Chemistry of Russian Academy of Sciences, Moscow, Russia
3 National Aviation University, Kyiv, Ukraine

The lipopolysaccharide from Escherichia coli L-19 was isolated, chemically identified and its nontoxicity but pyrogenicity were shown. The investigations of lipopolysaccharide influence on T- and B-lymphocytes indicated that it might be used as a mitogen in blasttransformation reactions since it was only in insignificant degree less active in comparison with the commercial one. It was shown that in reactions of Ouchterlony double immunodiffusion in agar LPS from E. coli L-19 exerted an activity of antigen in homological system. There were not established serological cross reactions between antiserum to heated E.coli L-19 cells and LPS from another of E.coli strain М-17 аnd also the representatives of Enterobacteriaceae species such as Budvicia aquatica, Rahnella aquatilis and Pragia fontium. These results indicate the absence of common antigenic determinants between the tested strains.

Кey words: Escherichia coli lipopolysaccharide, toxicity, pyrogenicity, mitogenic activity.

© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2015