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Home Archive 2015 № 1 SEROLOGICAL AND BIOLOGICAL ACTIVITY OF LIPOLYSACCHARIDE FROM ESCHERICHIA COLI L-19 L. D. Varbanets, E. A. Zdorovenko, Е. G. Garkavaya, O. S. Brovarskaya
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ISSN 2410-776X (Online),
ISSN 2410-7751 (Print)

Biotechnologia Acta
V. 8, No 1, 2015


Biotechnologia Acta, V. 8, No 1, 2015
doi: 10.15407/biotech8.01.032

P. 32-38, Bibliography 12, English
Universal Decimal classification: 579.841.11.22

SEROLOGICAL AND BIOLOGICAL ACTIVITY OF LIPOPOLYSACCHARIDE
FROM Escherichia coli L-19

L. D. Varbanets 1, E. L. Zdorovenko 2, Е. G. Garkavaya 3,  O. S. Brovarskaya 1

1 Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
2 Zelinsky Institute of Organic Chemistry of Russian Academy of Sciences, Moscow, Russia
3 National Aviation University, Kyiv, Ukraine

The lipopolysaccharide from Escherichia coli L-19 was isolated, chemically identified and its nontoxicity but pyrogenicity were shown. The investigations of lipopolysaccharide influence on T- and B-lymphocytes indicated that it might be used as a mitogen in blasttransformation reactions since it was only in insignificant degree less active in comparison with the commercial one. It was shown that in reactions of Ouchterlony double immunodiffusion in agar LPS from E. coli L-19 exerted an activity of antigen in homological system. There were not established serological cross reactions between antiserum to heated E.coli L-19 cells and LPS from another of E.coli strain М-17 аnd also the representatives of Enterobacteriaceae species such as Budvicia aquatica, Rahnella aquatilis and Pragia fontium. These results indicate the absence of common antigenic determinants between the tested strains.

Кey words: Escherichia coli lipopolysaccharide, toxicity, pyrogenicity, mitogenic activity.

© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2008

  • References
    • 1. Structure and biological activity of bacterial biopolymers. Ed. Posur V. K. Kyiv: «Kyivskyi universitet». 2003, 305 p. (In Russian).

      2. Varbanets L. D. Endotoxins of gram-negative: structure and biological activity. Mikrobiol. zh. 1994, 56 (3), 76–97. (In Russian).

      3. Hold G., Bryan C. The molecular basis of lipid A and Toll-like receptor 4 interactions. Eds. Y. A. Knirel, M. A. Valvano. Wien-N.Y.: Springer-Verlag. 2011, 433 p.
      http://dx.doi.org/10.1007/978-3-7091-0733-1_12

      4. Lucuk N. B. Biochemistry and immunology of microbial polysaccharides. Tomsk. 1984, 304 p. (In Russian).

      5. Varbanets L. D., Zdorovenko G. M., Knirel Yu. A. Methods of endotoxins investigation. Kyiv: Naukova dumka. 2006, 238 p. (In Russian).

      6. Sawardeker J. S., Sloneker J. H., Jeans A. Quatitative determination of monosaccharides as their alditol acetates by gas liquid chromatography. Anal. Chem. 1995, 37 (5), 1602–1603.

      7. Ouchterlony O. Diffusion in gel methods for immunological analysis. Prog. Allergy. 1962, N 6, P. 3–15.

      8. Belenkyi M. L. Elements of guantitative estimation of pharmacephtical effect. L.: Medtgis. 1963, 152 p. (In Russian).

      9. Seydel U., Wiese A., Schromm A., Brandenburg K. A biophysical view on the function and activity of endotoxins. In: Endotoxin in health and disease (Eds. Brade H., Opal S., Vogel S., Morrison D.). Marcel Dekker, Inc. New York, Basel. 1999, 999 p.

      10. Zdorovenko E. L., Varbanets L. D., Liu B., Valueva O. A., Wang G., Shashkov A. S., Garkavaya E. G., Brovarskaya O. S., Wang L., Knirel Yu. Structure of gene cluster of the O antigen of Escherichia coli L-19, a candidate for a new serogroup. Microbiology. 2014, V. 160, P. 2102–2107.
      http://dx.doi.org/10.1099/mic.0.080804-0

      11. Ostapchuk A. N., Varbanets L. D. Biological activity of native and modified Rahnella aquatilis lipopolysaccharides. Mikrobiol. zh. 2004, 66 (6), 31–36. (In Russian).

      12. Shubchinskyi V. V., Varbanets L. D., Brovarskaya O. S. Endotoxic activity of Pragia fontium lipopolysaccharides. Sovremennye problemy toxicologii. 2007, V. 4, P. 35–38. (In Ukrainian).

 

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Home Archive 2015 № 1 SEROLOGICAL AND BIOLOGICAL ACTIVITY OF LIPOLYSACCHARIDE FROM ESCHERICHIA COLI L-19 L. D. Varbanets, E. A. Zdorovenko, Е. G. Garkavaya, O. S. Brovarskaya

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