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Home Archive 2012 № 5 PHENOTYPE AND MIGRATION POTENTIAL OF MULTIPOTENT MESENCHYMAL STROMAL CELLS FROM NATIVE AND CRYOPRESERVED HUMAN PLACENTA V. A. Shablyy, M. D. Kuchma, V. M. Kyryk, G. M. Onishchenko, O. M. Tsupykov, P. P. Klymenko, P. O. Areshkov, O. V. Kuchuk, L. L. Lukash, G. S. Lobyntseva
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ISSN 1995-5537

Scientific journal

БІОТЕХНОЛОГІЯ"
V. 5, No. 5, 2012


«Біотехнологія» journal V. 5, No. 5, 2012
Р. 34-44, Bibliography 27, Ukrainian.
Universal Decimal classification: 601.2:575.853+57.86.13

PHENOTYPE AND MIGRATION POTENTIAL OF MULTIPOTENT MESENCHYMAL STROMAL CELLS FROM NATIVE AND CRYOPRESERVED HUMAN PLACENTA

V. A. Shablyy, M. D. Kuchma, V. M. Kyryk, G. M. Onishchenko, O. M. Tsupykov,
P. P. Klymenko, P. O. Areshkov, O. V. Kuchuk, L. L. Lukash, G. S. Lobyntseva

Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Kyiv

Institute of Cell Therapy, Kyiv

State Institute of Genetic and Regenerative Medicine of National Academy of Medical Science of Ukraine, Kyiv

Multipotent mesenchymal stromal cells of placental origin are used to treat critical limb ischemia, dilated cardiomyopathy, and hematopoiesis recovery after transplantation of hematopoietic stem cells. Therefore development of the methods of placental tissue cryopreservation and isolation of placental-derived multipotent mesenchymal stromal cells and the study of their molecular-biological properties and capacity to multilineage differentiation and regenerative potential is an actual task. It was shown that multipotent mesenchymal stromal cells excreted from native and cryopreserved human placenta tissue had similar morphology, immunophenotype and multilineage differentiation capacity. For the first time, it was established that placental multipotent mesenchymal stromal cells expressed mesenchymal and epithelial cells markers, contained of cytokeratin 7-positive cells that expressed vimentin and CD90. We isolated the cells that migrated from the placental tissue during culturing in cell culture medium with human serum that were similar in immunophenotype to the multipotent mesenchymal stromal cells. These multipotent mesenchymal stromal cells, isolated from native and cryopreserved placental, were able to survive homing and integrating into damage cardiac tissue of mice in vivo.

Key words: placenta, multipotent mesenchymal stromal cells, cryopreservation, cardiomyopathy, myocardium.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008

 

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Home Archive 2012 № 5 PHENOTYPE AND MIGRATION POTENTIAL OF MULTIPOTENT MESENCHYMAL STROMAL CELLS FROM NATIVE AND CRYOPRESERVED HUMAN PLACENTA V. A. Shablyy, M. D. Kuchma, V. M. Kyryk, G. M. Onishchenko, O. M. Tsupykov, P. P. Klymenko, P. O. Areshkov, O. V. Kuchuk, L. L. Lukash, G. S. Lobyntseva

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© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2008.
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