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Home Archive 2011 № 5 CONDUCTOMETRIC BIOSENSOR BASED ON ACETYLCHOLINESTERASE FOR CATIONIC SURFACTANTS DETECTION IN WATER SOLUTIONS I. S. Kucherenko, О. О. Soldatkin, V. M. Arkhypova1, S. V. Dzyadevych, A. P. Soldatkin
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ISSN 1995-5537

Scientific journal

БІОТЕХНОЛОГІЯ

Т. 4, № 5, 2011

"Biotechnology" journal V. 4, No. 5, 2011
Р. 83-89, Bibliography 11, Ukrainian.
Universal Decimal classification: 543.555+544.475

CONDUCTOMETRIC BIOSENSOR
BASED
ON ACETYLCHOLINESTERASE
FOR
CATIONIC SURFACTANTS DETECTION IN WATER SOLUTIONS

I. S. Kucherenko, О. О. Soldatkin, V. M. Arkhypova1, S. V. Dzyadevych, A. P. Soldatkin

Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics
of National Academy of Sciences of Ukraine, Kyiv;

Institute of Biology, Taras Shevchenko National University of Kyiv;

Institute of High Technologies, Taras Shevchenko National University of Kyiv

At the moment the ecological monitoring of environment took on special  significance. Permanent control of presence of toxic matters is foremost needed in an environment. One of dangerous toxicants of environment are surfactants. Conductometric biosensor for determination of surfactants has been developed for the

first time. As a conductometric transducer we used a differential pair of the planar  thin-film interdigitated electrodes deposited on the ceramic substrate. As bioselective element we used  acetylcholinesterase immobilized together with bovine serum albumin on the transducer’s surface by using glutaraldehyde. The optimal conditions for the acetyl cholinesterase immobilization in glutaraldehyde vapour were determined. Influence of characteristic of solution (ionic strength, pH and buffer capacity) on the work of developed biosensor was investigated. The biosensor’s sensitivity to benzalkonium chloride was tested. Good operational stability of the biosensor for determination of surfactants was shown. The possibility for determination of surfactant concentration in the water samples by using developed conductometric biosensor based on acetylcholinesterase was shown.

Key words: conductometric biosensor, acetylcholinesterase, surfactants, inhibitory analysis, environmental monitoring.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008

 

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Home Archive 2011 № 5 CONDUCTOMETRIC BIOSENSOR BASED ON ACETYLCHOLINESTERASE FOR CATIONIC SURFACTANTS DETECTION IN WATER SOLUTIONS I. S. Kucherenko, О. О. Soldatkin, V. M. Arkhypova1, S. V. Dzyadevych, A. P. Soldatkin

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© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2008.
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