GENOME ANALYSIS OF Pseudomonas brassicacearum S-1 – AN ANTAGONIST OF CROP PATHOGENS A. A. Muratova, A. E. Akhremchuk, L. N. Valentovich

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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

Biotechnologia Acta V. 14, No 2, 2021
Р. 47-58, Bibliography 37, English
Universal Decimal Classification:
https://doi.org/10.15407/biotech14.02.047

GENOME ANALYSIS OF Pseudomonas brassicacearum S-1 – AN ANTAGONIST OF CROP PATHOGENS

A. A. Muratova, A. E. Akhremchuk, L. N. Valentovich

Institute of Microbiology of the National Academy of Sciences of Belarus, Minsk

The strain Pseudomonas brassicacearum S-1 is the basis of the biopesticide “Ecogreen”, which is used to control pathogens infecting vegetable and green spicy crops in small-scale hydroponics.

Aim. The purpose of this work was to sequence and analyze the nucleotide sequence of the genome of strain P. brassicacearum S-1 (GenBank accession number CP045701).

Methods. Whole-genome sequencing was performed by both MiSeq (Illuminа) and MinION (Oxford Nanopore). Analysis of the genome sequence was performed with a number of bioinformatics programs.

Results. The genome of the P. brassicacearum S-1 strain comprising a single circular 6 577 561-bp chromosome with GC content of 60.8 %. Genome analysis revealed genes that constitute valuable biotechnological potential of the S-1 strain and determine synthesis of a wide range of secondary metabolites. Moreover, mobile genetic elements, prophages and short repetitive sequences were identified in the S-1 genome.

Conclusions. Detected genetic determinants, which are responsible for the synthesis of practically valuable compounds, indicate a significant potential of the P. brassicacearum S-1 strain as a biocontrol agent.

Key words: genome, sequencing, Pseudomonas brassicacearum, secondary metabolites, biocontrol.

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