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Home Archive 2021 № 6 TRANSFORMATION MEDIATED BY Agrobacterium rhizogenes AS APPROACH OF STIMULATING THE SYNTHESIS OF ANTIOXIDANT COMPOUNDS IN Artemisia absinthium L. A. I. Olkhovska, K. О. Drobot, A. M. Shakhovsky, N. A. Matvieieva
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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

Biotechnologia Acta  Т. 14, No. 6 , 2021
P. 60-70, Bibliography 24, Engl.
UDC: 543.544+547.655.6+577.1
https://doi.org/10.15407/biotech14.06.060

SIMULTANEOUS IDENTIFICATION, QUANTIFICATION, AND ANALYSIS OF MAIN COMPONENTS OF “HAIRY” ROOT EXTRACTS OF Artemisia annua AND Artemisia tilesii PLANTS

N. Kobylinska1, T. Bohdanovych2, V. Duplij2, I. Pashchenko3, N. Matvieieva1

1 Dumansky Institute of Colloid and Water Chemistry of of the National Academy of Sciences of Ukraine, Kyiv
2 Institute of Cell Biology and Genetic Engineering of of the National Academy of Sciences of Ukraine, Kyiv
3 Kyiv National University of Technologies and Design, Ukraine

Aim. The profiles of polyphenolic phytochemicals in extracts of “hairy” roots of Artemisia tilesii Ledeb. and Artemisia annua L. were studied. Analytical separation and quantification of main components in extracts were evaluated.

Methods. “hairy” roots were grown in vitro on Murashige and Skoog medium. High-performance chromatography coupled with different types of detection (photo diode array detection (DAD) and electrospray ionization with ultra-high resolution Qq-Time-of-Flight mass spectrometry) was used to identify and quantify the main biologically active components in ethanol extracts of “hairy” roots.

Results. The amount of flavonoids was 94.71–144.33 mg RE/g DW and 33.52–78.00 mg RE/g DW in “hairy” roots of A. annua and A. tilesii, respectively. In most samples of “hairy” roots, the amount of flavonoids was higher than the content in the control plant roots. The presence of Apigenin (0.168 ± 0.003 mg/L and 0.178 ± 0.006 mg/L), Quercetin (0.282 ± 0.005 mg/L and 0.174 ± 0.005 mg/L) in the extracts of A. annua and A. tilesii was shown by reverse-phase HPLC-DAD method. Chlorogenic acid, Kaempferol, and other flavonoids were detected.

Conclusions. The developed HPLC-DAD method demonstrated the high percentage of recovery, low limit of detection and quantification (9,11 ng/ml ≤ LOQ ≤16,51 ng/ml), accuracy and correctness. Thus, the method is suitable for the simultaneous quantification of phenolic acids and flavonoids in various plant extracts with short time and high efficiency.

Key words: Artemisia tilesii, Artemisia annua, polyphenols, flavonoids, “hairy” roots, reversed-phase HPLC with diode matrix detector.

© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2021

  • References
    • 1. Zhang B., Deng Z., Dan Ramdath D., Tang Y., Chen P. X., Liu R., Liu Q., Tsao R. Phenolic profiles of 20 Canadian lentil cultivars and their contribution to antioxidant activity and inhibitory effects on a-glucosidase and pancreatic lipase. Food Chem. 2015, V. 172, P. 862‒872. https://doi.org/10.1016/j.foodchem.2014.09.144

      2. Pietta P.-G. Flavonoids as antioxidants. J. Nat. Prod. 2000, 63 (7), 1035–1042. https://doi.org/10.1021/np9904509

      3. Shay J., Elbaz H. A., Lee I., Zielske S. P., Malek M. H., Hüttemann M. Molecular Mechanisms and Therapeutic Effects of (−)-Epicatechin and Other Polyphenols in Cancer, Inflammation, Diabetes, and Neurodegeneration. Oxid. Med. Cell. Longev. 2015, 181260. https://doi.org/10.1155/2015/181260

      4. Enogieru A. B., Haylett W., Hiss D. C., Bardien S., Ekpo O. E. Rutin as a Potent Antioxidant: Implications for Neurodegenerative Disorders. Oxid. Med. Cell. Longev. 2018, 6241017. https://doi.org/10.1155/2018/6241017

      5. Salehi B., Machin L., Monzote L., Sharifi-Rad J., Ezzat S. M., Salem M. A., Merghany R. M., El Mahdy N. M., Kılıç C. S., Sytar O., Sharifi-Rad M., Sharopov F., Martins N., Martorell M., Cho W. C. Therapeutic Potential of Quercetin: New Insights and Perspectives for Human Health. ACS Omega. 2020, 5 (20), 11849–11872. https://doi.org/10.1021/acsomega.0c01818

      6. Salehi B., Venditti A., Sharifi-Rad M., Kręgiel D., Sharifi-Rad J., Durazzo A., Lucarini M., Santini A., Souto E. B., Novellino E., Antolak H., Azzini E., Setzer W. N., Martins N. The Therapeutic Potential of Apigenin. Int. J. Mol. Sci. 2019, 20 (6), 1305. https://doi.org/10.3390/ijms20061305

      7. Ahmadi S. M., Farhoosh R., Sharif A., Rezaie M. Structure-Antioxidant Activity Relationships of Luteolin and Catechin. J. Food Sci. 2020, 85 (2), 298‒305. https://doi.org/10.1111/1750-3841.14994

      8. Ginwala R., Bhavsar R., Chigbu D. G. I., Jain P., Khan Z. K. Potential Role of Flavonoids in Treating Chronic Inflammatory Diseases with a Special Focus on the

      Anti-Inflammatory Activity of Apigenin. Antioxidants (Basel). 2019, 8 (2), 35. https://doi.org/10.3390/antiox8020035

      9. Wani H., Shah S., Banday J. Chemical composition and antioxidant activity of the leaf essential oil of Artemisia absinthium growing wild in Kashmir, Ind. J. Phytopharm. 2014, V. 3, P. 90–94, https://doi.org/10.31254/phyto.2014.3203

      10. Tu Y. From Artemisia annua L. to Artemisinins. The Discovery and Development of Artemisinins and Antimalarial Agents. Academic Press Agents, Elsevier. 2017. https://doi.org/10.1016/B978-0-12-811655-5/00027-1

      11. Bulgakov V. P. Functions of rol genes in plant secondary metabolism. Biotechnol. Adv. 2008, 26 (4). 318‒324. https://doi.org/10.1016/j.biotechadv.2008.03.001

      12. Shkryl Y. N., Veremeichik G. N., Bulgakov V. P., Tchernoded G. K., Mischenko N. P., Fedoreyev S. A., Zhuravlev Y. N. Individual and combined effects of the rol A, B, and C genes on anthraquinone production in Rubia cordifolia transformed calli. Biotechnol. Bioeng. 2008, 100 (1), 118‒125. https://doi.org/10.1002/bit.21727

      13. Ping L., Xu-Qing W., Huai-Zhou W., Yong-Ning W. High performance liquid chromatographic determination of phenolic acids in fruits and vegetables. Biomed. Environ. Sci. 1993, V. 6, P. 389‒398.

      14. Kečkeš S., Gašić U., Ćirković Veličković T., Milojković-Opsenica D., Natić M., Tešić Ž. The determination of phenolic profiles of Serbian unifloral honeys using ultra-high-performance liquid chromatography/high resolution accurate mass spectrometry. Food Chem. 2013, 138 (1), 32–40. https://doi.org/10.1016/j.foodchem.2012.10.025

      15. Jiang H., Engelhardt U. H., Thr ̈ane C., Maiwald B., Stark J. Determination of flavonol glycosides in green tea, oolong tea and black teaby UHPLC compared to HPLC. Food Chem. 2015, V. 183, P. 30–35. https://doi.org/10.1016/j.foodchem.2015.03.024

      16. Figueiredo-González M., Regueiro J., Cancho-Grande B., Simal-Gándara J. Garnacha Tintorera-based sweet wines: Detailed phenolic composition by HPLC/DAD–ESI/MS analysis. Food Chem. 2014, V. 143, P. 282–92. https://doi.org/10.1016/j.foodchem.2013.07.120

      17. Samanidou V., Tsagiannidis A., Sarakatsianos I. Simultaneous determination of polyphenols and major purine alkaloids in Greek Sideritis species, herbal extracts, green tea, black tea, and coffee by high-performance liquid chromatography-diode array detection. J. Sep. Sci. 2012, 35 (4), 608‒615. https://doi.org/10.1002/jssc.201100894

      18. Pekal A., Pyrzynska K. Evaluation of Aluminium Complexation Reaction for Flavonoid Content Assay. Food Anal. Methods. 2014, 7 (9), 1776–1782. https://doi.org/10.1007/s12161-014-9814-x

      19. ICH/2005/Q2/R1: ICH Validation of analytical procedures: Text and methodology. Q2 (R1). International Conference on Harmonization, Geneva, Switzerland. 2005.

      20. Pandey A. K., Singh P. The Genus Artemisia: a 2012‒2017 Literature Review on Chemical Composition, Antimicrobial, Insecticidal and Antioxidant Activities of Essential Oils. Medicines. 2017, V. 4, P. 68. https://doi.org/10.3390/medicines4030068

      21. Laghari A. H., Memon S., Nelofar A., Khan K. M., Yasmin A. Determination of free phenolic acids and antioxidant activity of methanolic extracts obtained from fruits and leaves of Chenopodium album. Food Chem. 2011, 126 (2011), 1850–1855. https://doi.org/10.1016/j.foodchem.2010.11.165

      22. Zeb M. A. Isolation and Biological Activity of β-Sitosterol and Stigmasterol from the Roots of Indigofera heterantha. Pharm. Pharmacol. Int. J. 2017, V. 5, P. 204–207. https://doi.org/10.15406/ppij.2017.05.00139

      23. Kasiri N., Rahmati M., Ahmadi L., Eskandari N. The significant impact of apigenin on different aspects of autoimmune disease. Inflammopharmacology. 2018, V. 26, P. 1359–1373. https://doi.org/10.1007/s10787-018-0531-8

      24. Galasso S., Pacifico S., Kretschmer N., Pan S. P., Marciano S., Piccolella S., Monaco P., Bauer R. Influence of seasonal variation on Thymus longicaulis C. Presl. chemical composition and its antioxidant and anti-inflammatory properties. Phytochem. 2014, V. 107, P. 80–90. https://doi.org/10.1016/j.phytochem.2014.08.015


 

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Home Archive 2021 № 6 TRANSFORMATION MEDIATED BY Agrobacterium rhizogenes AS APPROACH OF STIMULATING THE SYNTHESIS OF ANTIOXIDANT COMPOUNDS IN Artemisia absinthium L. A. I. Olkhovska, K. О. Drobot, A. M. Shakhovsky, N. A. Matvieieva

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